| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,225,954 | 0 | A | G | 64.7% | ‑3.0 / 14.5 | 17 | S41G (AGT→GGT) | yjbE | exopolysaccharide production protein YjbE |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (11/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATG > NZ_CP009273/4225875‑4226029 | tGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCcgc > 1:275565/1‑90 (MQ=255) cgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCa < 1:171945/90‑1 (MQ=255) ggCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCCCCCCCACCCCCGCCaccacccc > 1:358638/1‑88 (MQ=255) acaggTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTc < 1:625152/86‑1 (MQ=255) aGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCggggg > 2:258363/1‑90 (MQ=255) ggTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCCCCGGCCCACGGCAACACCCCAAACTCTGCgggggg > 1:327952/1‑90 (MQ=255) ggTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGGCCCGCGGCAGCACCCTAAGATCGGCgggggg > 1:227682/1‑90 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCCGCGTCAGCACCCTAAGCTCGGCgggggggg > 2:322152/1‑88 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGTCAGCACCCTAAGCTCGGCgggggggg > 2:443552/1‑88 (MQ=255) gggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGgggt > 2:94551/1‑90 (MQ=255) gggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTACTCCCCGCCCAGCGTCAGCCCCCGAAGCTCCggggggggggg > 2:320343/1‑87 (MQ=255) gggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCGCGACCAGCGTCAGCACCCGAAGCCCGGCggggggggg > 2:484368/1‑87 (MQ=255) ggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCCCCGGCCCGCGGCAGAACCCCGAGCCCGGCgggggggggc > 1:427201/1‑86 (MQ=255) aGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCt < 2:575628/90‑1 (MQ=255) gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGa < 2:232966/90‑1 (MQ=255) gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGa < 2:227682/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 2:358638/90‑1 (MQ=255) | TGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATG > NZ_CP009273/4225875‑4226029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |