| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,248,902 | 0 | T | G | 53.3% | 11.7 / 16.4 | 15 | G393G (GGT→GGG) | dinF | MATE family efflux transporter DinF |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCG > NZ_CP009273/4248815‑4248984 | cGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGttt < 2:604242/90‑1 (MQ=255) tGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGGTTTtcg > 2:187533/1‑90 (MQ=255) tgtTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTg < 2:567602/90‑1 (MQ=255) tATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTGACg < 1:552076/90‑1 (MQ=255) aTCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGGCGCCGCCGGGTTTTCGGTGACGc > 1:544206/1‑90 (MQ=255) gctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCCGGGTTTTCGCTGACGccccc > 2:374774/1‑89 (MQ=255) ggACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGGCGCCGCCGGGTTTTTGCTGGCGCCCCtttc > 2:552076/1‑88 (MQ=255) gCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGTGGCC‑CCGCCGGGGTTTGGCTGACGCGCCCTACGCTGc > 1:452390/1‑90 (MQ=255) gcgcAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGGGGCCCCCCCAGGGTTTTCGCCGACGCTCCCTACGCCGCCGtgggtggggg > 2:383218/1‑88 (MQ=255) gcAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGGCGCCGCCGGGTTTTGGCTGGCGCCCCTTTCGCCGCCGTGGGTGGGGAAt > 1:277204/1‑90 (MQ=255) gcAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGGGGCTGGGGAAt > 2:218009/1‑90 (MQ=255) ccGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggc < 2:277204/90‑1 (MQ=255) gAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGtggctg < 1:328048/90‑1 (MQ=255) gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTgcgc < 2:164863/90‑1 (MQ=255) gccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTgcgc < 2:340120/90‑1 (MQ=255) ccgcAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGGAATCATGCTTTGTGGCTGGCCTTTACCGTCTTTCTGGCGTTGcgcg > 1:327766/1‑90 (MQ=255) | CGTCTGGTGTTATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGC‑GGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCG > NZ_CP009273/4248815‑4248984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |