| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,203 | 0 | T | G | 56.2% | 4.6 / 16.8 | 16 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG > NZ_CP009273/642122‑642280 | ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAAAGc > 2:456839/1‑90 (MQ=255) gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTG‑GAAGGCCCCCcttttt > 1:47451/1‑89 (MQ=255) gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAATCCCCCCttttt > 2:317496/1‑90 (MQ=255) cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTcg < 1:661959/90‑1 (MQ=255) tcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCgcgc < 2:318574/90‑1 (MQ=255) cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTCAATAATGTTTTCGCCCCccgcc > 2:62270/1‑89 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgc < 2:561247/90‑1 (MQ=255) cTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAATGCCCCCCTTTTTTAATAATATTTTTGCCCCCCGCTGTGGCCAgcgc > 2:532576/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GAATGCCCCCCTTTTTTAATAAATTTTTTGCCCCCCGCCGTAGCCCGCGCa > 1:180273/1‑90 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGc < 1:532576/90‑1 (MQ=255) gAACTGCCGGAGATAATCGAGATCGGCGGGGGGTC‑GAATGCCCCCCTTTTTTAATAATGTTTTTGCCCCCCGCTGTAGCCCGCGCCGGcc > 2:578297/1‑90 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGccc < 1:578297/90‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGccc < 1:630867/90‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGccc < 2:117978/90‑1 (MQ=255) cTGCCGGAGATAATCGAGATCGGCGGGGGGTT‑GGATGCCCCCCTTTTTTAATAATATTTTTGCCTCCCGCCGTAGCCCGCGCAGGccccc > 1:659449/1‑90 (MQ=255) gaTCGGCGGGGGGTC‑GAATGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGGTg > 2:666453/1‑90 (MQ=255) | GGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTC‑GAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATG > NZ_CP009273/642122‑642280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |