| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 57.1% | 4.3 / 12.1 | 14 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGT > NZ_CP009273/1152642‑1152811 | aTACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGaaaa > 2:250086/1‑89 (MQ=255) tCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCgg < 1:363773/90‑1 (MQ=255) agaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACAt < 1:60672/90‑1 (MQ=255) gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATTTCGGGGTTTAAAACTCCttttt > 1:346866/1‑89 (MQ=255) cGGATTGCGGTGGCGTACTACACTTTTTTACAGAGGACAGAGAAACGGCGGGTAAATTTCTGGATCTCCGATTTAACCTCTCCTTTTCCg < 2:145992/90‑1 (MQ=255) gTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGa < 2:121641/90‑1 (MQ=255) aCAGAGGACAGAGAAACGGCGGGGAAAATAATGGGTCTCGGGGTTTTAATCTCCCTTTCCCGCGTTGTGACCTTCCCTTATACGGAGcga > 1:385960/1‑88 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 1:120236/90‑1 (MQ=255) gagGACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTAAATCTCCTTTTTCGGCATTTTGACCTTCCGTAATTCGGGGCAACTg > 2:175552/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTACATCTCCTTTTTCGGCCTTGTGACCTTCCGTAATGCGGGGCAAATgcg > 2:389028/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTAAATCTCCTTTTTCGGCCTTGTGGCCTTTCCCAAATCGGGGCAACTgcg > 2:34969/1‑90 (MQ=255) gagaAACGGCGGGGAAATTTCTTGGTGTCGGGGTTTTTATCTCCTTTTTCTGCGTTGTGACCTTTCGGAATTCGGAGGAAATGCGCGGtg > 1:357243/1‑90 (MQ=255) gagaAACGGCGGGGAAATTACTGGGTCTCCGATTTTAAATCTCCTTTTTCCGCATTGTGGCCTTCCCTAATTCGGAGGAACTGCGCGAtg > 2:557229/1‑90 (MQ=255) gcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGt < 2:313678/90‑1 (MQ=255) | ATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGT > NZ_CP009273/1152642‑1152811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |