Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,622,3070TG55.6% 11.3 / 16.7 18G178G (GGT→GGGydfGbifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base G (10/0);  total (14/4)
Fisher's exact test for biased strand distribution p-value = 2.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG  >  NZ_CP009273/1622224‑1622394
                                                                                   |                                                                                       
aaaGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGGGCGTCACCCACATCGAACCGGGGCtgggg                                                                                   >  2:309793/1‑88 (MQ=255)
  aGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTgggggg                                                                                 >  2:212984/1‑90 (MQ=255)
        tGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTAc                                                                           <  1:498264/90‑1 (MQ=255)
          ttCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCg                                                                         <  1:309793/90‑1 (MQ=255)
             gTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGCCACCCACATCGAACCGGGGCTGGTGGGGGGGACCGAGt                                                                      >  2:604885/1‑90 (MQ=255)
                                        aTCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATg                                           <  1:270899/90‑1 (MQ=255)
                                          cTGCATGGTACGGCGGTGCGCGTCACCGACATCGAAACGGGGCTGGTGGGGGGGACCGGGGTTTCCCATGTCCGCCTTAAAGGCGATGAc                                         >  1:228789/1‑90 (MQ=255)
                                                 gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGTGGTACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAg                                  >  1:307422/1‑90 (MQ=255)
                                                 gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGGGGTTTCCAATGTCCCCTTTAAAGGCGGTGACGGGAAAg                                  >  1:591045/1‑90 (MQ=255)
                                                 gTACGGCGGTGCGCGTCACCGACATCGAAACGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCGCTTTTAAGGCGGTGACGGGAAAg                                  >  1:108534/1‑90 (MQ=255)
                                                 gTACGGCGGTGCGCGGCACCGACATCGAAACGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCCCTTTTAAAGCGATGACGGGaaaa                                  >  1:130493/1‑89 (MQ=255)
                                                  tACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGc                                 <  2:222354/90‑1 (MQ=255)
                                                      gcggTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGaa                             >  1:100209/1‑90 (MQ=255)
                                                           gcgcGTCACCGACATCGAACCGGGTCTGGTGGGGGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc                        >  2:147641/1‑90 (MQ=255)
                                                           gcgcGTCACCGACATCGAACCGGGTCTGGTGGGGGGTACCGAGGTTTTCAATGTCCGCTTTAAAGGGGGTGACGGGAAAGCAGAAAAAAc                        >  1:455580/1‑90 (MQ=255)
                                                           gcgcGTCACCGACATCGAACCGGGGCTGGTGGGGGGTACCGGGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc                        >  1:222354/1‑90 (MQ=255)
                                                                                 ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg  >  1:382973/1‑90 (MQ=255)
                                                                                 ggTCTGGTGGGTGGTACCGAGTCTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg  >  1:99516/1‑90 (MQ=255)
                                                                                   |                                                                                       
AAAGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG  >  NZ_CP009273/1622224‑1622394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: