Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,300,6280TG52.9% 31.1 / 42.6 34T198P (ACC→CCC) deaDATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base T (6/10);  new base G (18/0);  total (24/10)
Fisher's exact test for biased strand distribution p-value = 6.11e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-06
Rejected as consensus: Frequency below/above cutoff threshold.

AGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACGTCTT  >  NZ_CP009273/3300542‑3300717
                                                                                      |                                                                                         
aGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAAt                                                                                        >  1:10958/1‑90 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATg                                                                                       >  1:14817/1‑90 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGaaag                                                                                       >  2:526180/1‑88 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGaaag                                                                                       >  2:412466/1‑88 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATg                                                                                       >  1:240314/1‑90 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATg                                                                                       >  1:392347/1‑90 (MQ=255)
 gTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATg                                                                                       >  2:105553/1‑90 (MQ=255)
      gTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGcgacg                                                                                  >  2:259410/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGGGACGAAGCg                                                                             >  1:364061/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCg                                                                             >  1:184877/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGGGACGAATCg                                                                             >  2:553910/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCg                                                                             >  2:566537/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCg                                                                             >  2:53751/1‑90 (MQ=255)
           tctGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTCTCATAAAGCGGCGGGGAATGGGAAGGATCg                                                                             >  1:518686/1‑90 (MQ=255)
              ggCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCtt                                                                          >  1:609922/1‑90 (MQ=255)
                    tGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCa                                                                    >  2:11495/1‑90 (MQ=255)
                     gTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCAt                                                                   <  2:14817/90‑1 (MQ=255)
                        aGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAAAGCGACGAATCGCTTCCGGCATTGt                                                                >  2:358087/1‑90 (MQ=255)
                             gggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCag                                                           <  2:485843/90‑1 (MQ=255)
                             gggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCag                                                           <  2:184877/90‑1 (MQ=255)
                              ggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCCTGGTTGCaga                                                          >  1:4815/1‑90 (MQ=255)
                              ggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAAACGCTTCCGGCATGGTTGCaga                                                          >  1:93613/1‑90 (MQ=255)
                              ggTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAAAGCGACGAATCGCTTCCGGCATGGGTGCagg                                                          >  1:358646/1‑89 (MQ=255)
                                  gTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAAc                                                      <  2:494520/90‑1 (MQ=255)
                                        cTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCg                                                <  1:358087/90‑1 (MQ=255)
                                               gAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCtgat                                         >  1:74200/1‑90 (MQ=255)
                                                 aTGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCtgatga                                       <  2:392347/90‑1 (MQ=255)
                                                         ttCCTGCGGCTCTTTCATAAAGCGGCGGGGAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCgg                               >  1:81565/1‑90 (MQ=255)
                                                                       tCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATaa                 <  1:317553/90‑1 (MQ=255)
                                                                           aaaGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCag                                                           <  2:379886/44‑1 (MQ=255)
                                                                           aaaGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCag                                                           >  1:379886/1‑44 (MQ=255)
                                                                              gcggcgGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCg                                                >  1:67087/1‑52 (MQ=255)
                                                                              gcggcgGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCg                                                <  2:67087/52‑1 (MQ=255)
                                                                                 gcggGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAAc       <  1:53751/90‑1 (MQ=255)
                                                                                      tAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACGTCtt  <  2:57035/90‑1 (MQ=255)
                                                                                      |                                                                                         
AGTCCAGTAGCTCTGGCTGATGTCAGGACGGGTAGTCACGCTGGACTGAATGCGCACTTCCTGCGGCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACGTCTT  >  NZ_CP009273/3300542‑3300717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: