Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	381,253	0	T	G	56.2%	8.6 / 17.1	16	V189G (GTG→GGG)	tauA	taurine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base G (9/0);  total (11/5)
Fisher's exact test for biased strand distribution p-value = 4.81e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCT  >  NZ_CP009273/381171‑381306                                                                                   |                                                      aTCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAcc                                                <  1:173396/90‑1 (MQ=255) aTCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAcc                                                <  1:544837/90‑1 (MQ=255)      tGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGAAAAAAACGGCAAGGGGGTGACCCAttt                                           >  2:302737/1‑89 (MQ=255)                                gTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGTCGGGCAGTGGGgggcgc                 >  2:46331/1‑90 (MQ=255)                                gTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGTCGGGCAGGGGGgggcgc                 >  2:217311/1‑90 (MQ=255)                                gTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCTGGAAAAAAACGGGAAGGGGTTGACCGATTTTGAACAGGTCGGGCAGTGGGgggcgc                 >  2:610085/1‑90 (MQ=255)                                 tGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACa                                     <  1:84228/69‑1 (MQ=255)                                 tGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACa                                     >  2:84228/1‑69 (MQ=255)                                  gCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGGAAAAAACGGCAAGGGGTTGACCGATTCTGAAAAGGGCGGGCAGTGGGGCGCGCCa               >  1:244894/1‑90 (MQ=255)                                  gCTTATGTCTGGGCACCGGCGGTTAACGCCCCGGGAAAAGACGGCAAGGGGTTGGCCGGTTTTGAACAGGGCGGGGGGGGGGGGGCGCCa               >  1:177231/1‑90 (MQ=255)                                          cTGGGCACCGGCGGTTAACGCCCCGGAAAAAAACGGCAAGGGGTTGGCCGAGTCTGAACAAGGCGGGCGGTGGGGGGGGCCAACGCTggg       >  1:612521/1‑89 (MQ=255)                                           tGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGGCGGGCAGTGGGGGGCGCCAACGCTgggc      >  1:183938/1‑88 (MQ=255)                                            gggCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGAc      <  1:155575/89‑1 (MQ=255)                                            gggCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGAc      >  2:155575/1‑89 (MQ=255)                                            gggCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGTCGGGCAGGGGGGCGCGCCAACGCTGGACg     >  2:229766/1‑90 (MQ=255)                                               cACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGCCGGGCAGTGGGGCGCGCCAACGCTGGACGTCt  <  1:403039/90‑1 (MQ=255)                                                                                   |                                                      ATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCT  >  NZ_CP009273/381171‑381306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑