Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,387,0300AG54.5% 5.9 / 10.3 11L863P (CTC→CCC) yhdPAsmA2 domain‑containing protein YhdP
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAA  >  NZ_CP009273/3386942‑3387103
                                                                                        |                                                                         
aGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAg                                                                          <  2:387579/90‑1 (MQ=255)
                   gccagccaCTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTGTCTgccgcc                                                       >  2:284904/1‑90 (MQ=255)
                                     ttCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTGTCTGCCGCCCCAAAAACACGATctttc                                     >  2:102057/1‑86 (MQ=255)
                                        aTCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTc                                  <  2:76093/90‑1 (MQ=255)
                                                       aGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTGTTTTCCGCCCCAAAAAAACCAACCAGCGTCAGCTTTTTTCCGAGc                   >  1:437394/1‑90 (MQ=255)
                                                             aCGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTGTCTGCCCCCCAAAAAACACGATCGAGCGGCAGCTTTTTACCGCGCAACCAg             >  1:274659/1‑90 (MQ=255)
                                                             aCGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTTCTGTCTGCCGCCCAAAAAGCACGATCGAGCGTCAGCTTTTTACCGAGCAACCAg             >  2:162729/1‑90 (MQ=255)
                                                               gCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCg           <  1:412005/90‑1 (MQ=255)
                                                                ccACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTGTCTTCCCCCCCAAAAACACGATCCAGCGGCCGCTTTTTTCCCAGCAACCAGCgg          >  1:440403/1‑90 (MQ=255)
                                                                  aCTTTGTTCCGGCAACGGCGGGGGCGGTTTACTTGCTGGCGCCCAAAAAGCACCGACGAGCGTCAGGTTTTTAGCGGGCAAACAGTgggt        >  2:93198/1‑88 (MQ=255)
                                                                        ttCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGaa  <  2:44363/90‑1 (MQ=255)
                                                                                        |                                                                         
AGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAA  >  NZ_CP009273/3386942‑3387103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑