| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 431,960 | 0 | A | C | 53.8% | 8.1 / 14.1 | 13 | T291P (ACC→CCC) | thiL | thiamine‑phosphate kinase |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATA > NZ_CP009273/431886‑432045 | aGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTAcc < 1:341370/77‑1 (MQ=255)aGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTAcc > 2:341370/1‑77 (MQ=255) tttttCACTGTGCCGGAACTGACCCGGGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCGTTTCCCTGTATCGGGCAAATGAcc < 2:209939/88‑1 (MQ=255) ttCCCTGTGCCGAAACGGACCGGGGGCGCGGGGGATGGGCCTCTCGGACACCTGGGCGTCCCTTTTCCCTGTATCGGGCAAATGAccgcc < 2:52169/90‑1 (MQ=255) gtgCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATc > 2:167735/1‑90 (MQ=255) gCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGa > 1:272840/1‑90 (MQ=255) ccGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGaa > 2:538057/1‑90 (MQ=255) ccGGGGCGCGTTGGATGTGCCTCTCGGACCCGGGGGCGTCCTTTTTCCCTTTAGCGGGAAAATGCCCGCCGATATCGAAGGGCTTTGttt < 2:272840/90‑1 (MQ=255) tctgGGACACCTGGGCGTCCCTTTTCCCTGTATGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGt < 1:538057/90‑1 (MQ=255) tctcGGCCACCTGGGCGTACCTTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGt < 1:202555/90‑1 (MQ=255) ctcGGACCCCGGGGCGTCCCTTTTCCCTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGtt < 1:508822/90‑1 (MQ=255) cctACCGTTTCCCTGTACCGGGAAAATGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaa < 2:552375/88‑1 (MQ=255) gTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGata > 1:144312/1‑90 (MQ=255) | AGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATA > NZ_CP009273/431886‑432045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |