| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,048 | 0 | T | G | 64.7% | 3.1 / 13.8 | 17 | V81G (GTC→GGC) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (11/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAC > NZ_CP009273/831962‑832130 | gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:638255/1‑90 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 1:320576/1‑90 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCAc > 1:138476/1‑90 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCAc > 2:246538/1‑90 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCg > 1:29068/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCGGTGGGGGTCGGGCATTTTGGCAg > 1:423241/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTTGGCAg > 1:131415/1‑90 (MQ=255) aaCCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACCCCCCATGGCGGACGCCCCTTTTGTGAGGGGGCGGGGCACGAAggg > 2:574767/1‑88 (MQ=255) tGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCt < 1:32606/90‑1 (MQ=255) ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg < 2:528371/90‑1 (MQ=255) aaaCCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGATGGGGATCGCGCCTTTGGTCAGGGCGCGGCACATGAAACGATGGGGGTggggg > 2:84995/1‑89 (MQ=255) aCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGAtt < 1:536965/90‑1 (MQ=255) gTTAAAGAGGCGGGGGGGGGGGGCACGCTCGATGGCGATCGCGCATTTTGGCAGGGCCCGGCACATGAAGCGAAGGGGCTGGGGGTTgag > 1:315346/1‑90 (MQ=255) ttAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGCACATGAAGCGATGGCGCTGGGGGTTgaga > 1:216532/1‑90 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 2:131415/90‑1 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 2:320576/90‑1 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 1:422243/90‑1 (MQ=255) | GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAC > NZ_CP009273/831962‑832130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |