Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,504,6520CA55.6% 4.8 / 13.5 9S131R (AGC→AGA) ydcRPLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (0/5);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTC  >  NZ_CP009273/1504569‑1504713
                                                                                   |                                                             
caggtctTTCCTTTTGCCCCGCCCTTCCCCGCCCCGCGACTTTCCCCCCCCCAACAACAAACCCGCTCGCTGGCGCAGGTAAGAAAAAcc                                                         <  2:27828/88‑1 (MQ=255)
             ttGTCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGactg                                            >  1:27828/1‑87 (MQ=255)
                 cccGGCCTTCCCCCCCCCGCGATTTTCCCCCCCCCAACAACAAACCCGCCCGCGGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGt                                        <  1:280077/88‑1 (MQ=255)
                                   gcgACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGcc                      >  2:168992/1‑90 (MQ=255)
                                   gcgACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGcc                      >  2:379774/1‑90 (MQ=255)
                                   gcgACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTAACGcc                      >  2:127104/1‑90 (MQ=255)
                                                 ccaaacaacAAACCCCCCCGCTGGCGCAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAAc        <  1:168992/87‑1 (MQ=255)
                                                  caaacaacAAACCCGCTCGTGGGCCCAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACt       <  1:322600/88‑1 (MQ=255)
                                                       aacAAACCCGCTCGCGGGCGCAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTc  <  1:226658/90‑1 (MQ=255)
                                                                                   |                                                             
TCGGTCGTTCCGTTTGCCTCGGCCTTTCCCGACCCGCGACTTTTCCCCCTCCAACAACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTC  >  NZ_CP009273/1504569‑1504713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑