| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,257 | 0 | T | G | 52.9% | 9.5 / 16.2 | 17 | Y557S (TAC→TCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATT > NZ_CP009273/1548169‑1548343 | gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTa > 2:95123/1‑90 (MQ=255) gagaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc < 1:95123/90‑1 (MQ=255) gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCgg > 1:515528/1‑90 (MQ=255) gagaGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTGCCGGGATTTGGCTTTCCCGAAAtt > 1:350070/1‑90 (MQ=255) cccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGcc < 2:178679/90‑1 (MQ=255) ccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCt > 1:122348/1‑90 (MQ=255) cTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTTCAgg > 1:36751/1‑90 (MQ=255) tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTGGCTTTCCAGAAAATATCGTCAATGGCCTTTTGCAGGGc > 1:622574/1‑90 (MQ=255) aTGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGcc < 1:431426/90‑1 (MQ=255) ggAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCAc < 2:122348/90‑1 (MQ=255) gAGGTACGGCGGTAGTCGCGGTGTTCGGCTTCCCGGAAATTATCGGCAAGGGCCTGTTGGAGGGTTTCGGGCGAAGATTTCACCACCCCg > 2:575328/1‑90 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTTGGCTTTCCAGAAAATATCGTCAATGGGCTGTTGCAGGGGTTCGGCAGAGGGTTTCACccccccc > 2:431129/1‑87 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAAATATCGTCAATGGCCTGTTGCAGGGGTTCGGCAGAGGGTTTTACCCCCACg > 2:143967/1‑90 (MQ=255) gAGGTACGGCGGTAGGCGCGGGATTTGGCTTGCCCGAAAATATCGTCAATGGCCTGTTGCAGGGGTTTGGGAGAGGGTTTTACCGccccc > 2:118506/1‑87 (MQ=255) aGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGc < 1:431129/90‑1 (MQ=255) aGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCCGAAATTATTGGCAAAGGGCCGTTGGAGGGGTTCGGGAGAGGGTTTTACcccccccc > 2:384361/1‑86 (MQ=255) cgGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCAtt > 1:544916/1‑90 (MQ=255) | GATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATT > NZ_CP009273/1548169‑1548343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |