| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,285,537 | 0 | A | C | 54.5% | 7.0 / 10.1 | 11 | V118G (GTC→GGC) | ccmH | cytochrome c‑type biogenesis thiol:disulfide oxidoreductase CcmH |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTG > NZ_CP009273/2285449‑2285626 | atatCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAc > 1:466535/1‑90 (MQ=255) ccccGGCCCGCTTCCCTCCCGGCCCGTTTTGTCCAGAAAACCCTCCCGGCCCCCCGCGTCCCCGCCGCCGCGAGCGGGCGTATATGcccc < 2:298171/88‑1 (MQ=255) ccGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAg > 1:445451/1‑90 (MQ=255) aCGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCact > 1:562269/1‑90 (MQ=255) ttGTTCAGGAACCGCTCCCGCCCCCCCGCGCCCCCGCCCCCGCGGGGGGGCTTATATCCCCCACCCGCCAATGCCAATAGCCACTACTgg < 2:600931/90‑1 (MQ=255) cGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAcag > 1:171784/1‑90 (MQ=255) cGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAcag > 1:331785/1‑90 (MQ=255) cgcgTCCCCGACGCCGCGAGCGGGCGTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCGgcg < 1:47436/90‑1 (MQ=255) cgccgcGGGGGGGCTTATAGGCCCCACCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAAcggc < 2:562269/90‑1 (MQ=255) ccccgcGGGGGGGGGTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAAcggc < 2:171784/88‑1 (MQ=255) gCGGGCGTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTa < 1:358571/90‑1 (MQ=255) gTATAGGCCCCACCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGAc < 1:627362/90‑1 (MQ=255) aCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAgcg < 1:168122/58‑1 (MQ=255) aCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAgcg > 2:168122/1‑58 (MQ=255) aCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGGAAGTGACGAAGTTg > 1:156382/1‑90 (MQ=255) | ATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTG > NZ_CP009273/2285449‑2285626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |