| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,636,048 | 0 | T | G | 54.5% | 5.6 / 11.0 | 11 | A52A (GCA→GCC) | rodZ | cytoskeleton protein RodZ |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635963‑2636137 | gcagcaGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCt < 1:237639/90‑1 (MQ=255) ctnctGGAATATGTACCAGACGCGCATAANAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCaa < 1:140065/90‑1 (MQ=255) ccAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGGGCCTTTTCTTTTTTAATTTCGCGGACCgggg > 1:172600/1‑90 (MQ=255) aGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGCGGGGGGCCTTTTTTTTTTC‑ATTTTGCGTTCCGTggga > 2:504195/1‑88 (MQ=255) aGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGTACCGTGGaa < 1:635933/90‑1 (MQ=255) gCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGGGCCTTTTCTTTTTTAATGTCGCGTGCCGTGGGAAACTTCAGGCAAAg > 1:286778/1‑90 (MQ=255) gcgcAGGAATGTTGAAGCAAGATCGGC‑GGGGGGCTTTTTTTTTTTTTTTTCGCGTTCCGGGGGAACCTTCCCGCAAAGAAGCTCGGCCAc > 2:443809/1‑90 (MQ=255) cgcAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACg < 2:286778/90‑1 (MQ=255) gAAGCAAGATCGGC‑GGGGGCCTTATCTTCTTCAATGTCGCGTACCGGGGAAAACTTCAGGCAAAAACGCCCGGCAACGGCCCGCTGACTa > 1:230339/1‑90 (MQ=255) aaGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTaa < 2:364701/90‑1 (MQ=255) aGCAAGATCGGC‑GGGGGCCTTTTTTTCTTCCATGTTGCGTACCGTGGGAAACTTCAGGCAAAATCGCTCCGCAACGGCCCGCTGGCTAAg > 1:412948/1‑90 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 1:19258/90‑1 (MQ=255) tGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTcgcg < 2:412948/90‑1 (MQ=255) | GCAGCAGTTCTTCTTCTGGAATATGTACCAGACGCGCATAAGAGCGGATATATCCGCGCAGGAATGTTGAAGCAAGATCGGC‑GGGTGCCTTATCTTCTTCAATGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCG > NZ_CP009273/2635963‑2636137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |