Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,390,9090AC53.8% 6.1 / 11.9 13G82G (GGT→GGG) rngribonuclease G
Reads supporting (aligned to +/- strand):  ref base A (5/1);  new base C (0/7);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAA  >  NZ_CP009273/3390834‑3390976
                                                                           |                                                                   
caccTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTc                                                       >  2:276795/1‑90 (MQ=255)
  ccTCCACCACAAGACCTTGCCCCAGACGAACCACTTCCGAGATGTCGCCCCCCGTAAATTGTTTTTGTTCTCCACCCGCCACACATTCgg                                                     <  1:490242/90‑1 (MQ=255)
                   tGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCg                                    >  2:386221/1‑90 (MQ=255)
                     ccccGGACGACCCAGTCCGGGGAGGCCGCCCCCCGTAATTTTTTTTTTTTCTCCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGa                                  <  2:555305/90‑1 (MQ=255)
                         tGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGGGCGGCATGATGTCGGATGCa                              >  1:610256/1‑90 (MQ=255)
                               aCCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATgaaga                        >  1:158244/1‑90 (MQ=255)
                                    ttCCGAGATGTCGCGCCCCGTGAATTGCTTTTGTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc                   <  1:47685/90‑1 (MQ=255)
                                    tcccGAGATGTCGCGCGCTGTAATTTTTTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc                   <  2:412127/88‑1 (MQ=255)
                                              tCGCGCCCCGTGAATTGTTTTTGTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATcc         <  1:157597/90‑1 (MQ=255)
                                              ccgcgCCCCGTGAATTGTTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATcc         <  1:287866/89‑1 (MQ=255)
                                                     ccGTGAATTGTTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCaa  <  1:193017/90‑1 (MQ=255)
                                                       gTGAATTGCTTTTGTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCtt              <  1:26447/76‑1 (MQ=255)
                                                       gTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCtt              >  2:26447/1‑76 (MQ=255)
                                                                           |                                                                   
CACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAA  >  NZ_CP009273/3390834‑3390976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑