Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,578,4460AC61.5% ‑0.7 / 10.0 13Y580D (TAC→GAC) ggtgamma‑glutamyltransferase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/8);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCC  >  NZ_CP009273/3578360‑3578533
                                                                                      |                                                                                       
tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc                                                                                      >  1:331644/1‑90 (MQ=255)
tACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTAccc                                                                                      >  2:632583/1‑90 (MQ=255)
 aCAGGATTACAAAAAAAAAAAGGCTCCCTTCGGCTTGCCCTAAAAAAATACCCCTCTCCCCACGAAGGGGGCCGCTAACCTTAGTCcccc                                                                                     <  2:203040/90‑1 (MQ=255)
        agagAAAAAAAAAAGGCTCCCTTCGGCTTCCCCTGAAAAAATACCCCTCTTCCCACGAAGGGGGCCGCTAACTTTAGTCCCCCGCCGTTa                                                                              <  1:629630/87‑1 (MQ=255)
                  aaaaGGCCCCCTCCGCCTTCCCCTAAAAAAACCCCCCTCTCCCCACGAAGGGGGCCGCTACCCTTACTCCCCCGCCGTTAAATCATCCAc                                                                    <  2:331644/90‑1 (MQ=255)
                        cTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAgcg                                                              >  2:364937/1‑90 (MQ=255)
                                                             caggAAGGGGGCCGCAACCCTAAGACCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCa                         <  1:306379/87‑1 (MQ=255)
                                                             cacaAAGAGGCCCGCTACCCTTAGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCa                         <  1:49815/90‑1 (MQ=255)
                                                               cGAAGGGGGCCGCTACCCTAATTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAAc                       <  1:538334/90‑1 (MQ=255)
                                                                     gggCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAAt                 >  1:18346/1‑90 (MQ=255)
                                                                           ctccccTTTGTCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCCCCGTCCGGCCCAACCATAATGCTTtg           <  1:632583/86‑1 (MQ=255)
                                                                           cTACCCTTAGCCCCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTtg           <  1:580022/90‑1 (MQ=255)
                                                                                    gTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGcc  >  2:450771/1‑90 (MQ=255)
                                                                                      |                                                                                       
TACAGGATTACGAAATAAAAAAGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACCTTAGTACCCCGCCGTTAAATCATCCACCGAGCGCGGGTCGGATGCGCCGTACAACTCACCGTCCGGCCCAACCATAATGCTTTGTGTACTGCC  >  NZ_CP009273/3578360‑3578533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: