| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 52.6% | ‑2.2 / 15.3 | 19 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/3); new base A (0/10); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058885‑4059038 | ccAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCACGCCa > 2:613463/1‑90 (MQ=255) aaTATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCACGCCATa < 1:613463/90‑1 (MQ=255) tttttcCCGCGAGCCGAAAAAAAATCCGGTGAGCATATCGGTAAACGGGGAAAAAACCTTCCCCACCAAAAAGATAATCCCGCCATAGTa < 1:391375/89‑1 (MQ=255) tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGc > 2:102430/1‑90 (MQ=255) gcgaggcgagAAAAAACCCGGTGAGCATATCGGAAAACGCGGAAAAAAACTTCCCCACCAGAAAGATAACCCCGCCATAGTAAGCAGGCa < 1:629162/90‑1 (MQ=255) gcgAGTGGAGGAAAAACCCGGTGAGCATATCGGAAACCGCGGTAAAAACCTTCCCCCCAAAAAAGAAAACCCCGCCATAGTAAGCAGGCa < 1:271527/90‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:289051/1‑90 (MQ=255) gagtcgagtAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATc > 2:613586/1‑90 (MQ=255) gagtcgaaaAAAAATCCGGTGAGAATATCGGAAAACGCGGAAAAAACCTTCCCCCCAAAAAAGATAATCCCGCCATAGTAAGCAGGCATc < 1:613586/90‑1 (MQ=255) tggatAACCGCGAAAAAAAACTCCCCCACAAAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 2:547237/86‑1 (MQ=255) tCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg > 1:465254/1‑90 (MQ=255) gggaaaaCGCGGAAAAAACCTCCCCCACAAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:440126/86‑1 (MQ=255) cGGTAAACGCGGAAAAAACCTCCGCCACCAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:142827/90‑1 (MQ=255) cGGAAAACGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:168412/90‑1 (MQ=255) gTAACCGGGGAAAAAACCCCCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 1:377936/90‑1 (MQ=255) gcgGAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACaga < 2:532478/90‑1 (MQ=255) gaaaaaaacCTCCGCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt < 2:465254/87‑1 (MQ=255) aaaaaaCTCCCCCACAAGAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:222657/90‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:641409/1‑90 (MQ=255) | CCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058885‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |