Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,501,8650AC53.8% 5.5 / 11.6 13L123V (TTG→GTG) fecDFe(3+) dicitrate ABC transporter permease subunit FecD
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (0/7);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAG  >  NZ_CP009273/4501788‑4501945
                                                                             |                                                                                
gCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATgcc                                                                      >  1:614979/1‑90 (MQ=255)
 cATGCAAAAGGCGCCACGCGGGTGAGCGCCAGCTTCATCGGCTGGGGGGTTTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATgccg                                                                     <  2:84213/90‑1 (MQ=255)
        aaaGCCCCCCGCCGGTGAGCCCCACCTTCCTCGGCTGGTGGGTTTTTGCCAGCATCTCCAGCAATATCAACCCCGCCATGCCGCCCGCaa                                                              <  2:253938/90‑1 (MQ=255)
              ccACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGccg                                                        >  1:11529/1‑89 (MQ=255)
                 cgcgGGTGAGCGCCAGCTTCATCGGCGGGGGGTTTTTTCCCAGCATCTTCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGGCcagca                                                     <  2:531058/90‑1 (MQ=255)
                                 cTTCATCGGCTGGGGGGTTTTTGCCAGCATCTTCAGCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCAc                                     <  1:616194/90‑1 (MQ=255)
                                    cATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACggg                                  >  2:5442/1‑90 (MQ=255)
                                     aTCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGc                                 <  1:5442/90‑1 (MQ=255)
                                             gtggTTTTTTGCCAGCTTCTTCAGAAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACgg                         <  2:131037/90‑1 (MQ=255)
                                                    ttggCCAGCATCTTCAGCAATACCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATaaga                  <  2:614979/87‑1 (MQ=255)
                                                        ccAGCATTTTCAGAAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTa              <  1:567732/90‑1 (MQ=255)
                                                           gCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTagag           >  1:276498/1‑90 (MQ=255)
                                                                    gCAATATCACCCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACag  <  1:45876/90‑1 (MQ=255)
                                                                             |                                                                                
GCATGCAGAAAGCGCCACGCCGGTGAGCGCCAGCTTCATCGGCTGGTGGGTCTTTGCCAGCATCTTCAGCAATATCAACCCCGCCATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAG  >  NZ_CP009273/4501788‑4501945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑