Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,627	T→A	V84E (GTG→GAG)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,627	0	T	A	100.0%	40.1 / NA	13	V84E (GTG→GAG)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/2);  total (11/2)

CTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCC  >  NZ_CP009273/1613543‑1613710                                                                                     |                                                                                    cTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGaaa                                                                                >  1:97146/1‑90 (MQ=255)   ccGGTTGAACTGAAAAAGGGATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAgg                                                                              >  2:513587/1‑90 (MQ=255)     ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGtt                                                                            >  1:263647/1‑90 (MQ=255)          aaCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGa                                                                       <  2:585123/90‑1 (MQ=255)                   aGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATg                                                              >  1:558265/1‑90 (MQ=255)                    ggTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGa                                                             >  1:263208/1‑90 (MQ=255)                                        gAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTaa                                         <  2:97146/90‑1 (MQ=255)                                           cACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAAc                                      >  1:568391/1‑90 (MQ=255)                                                         cTGGATCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCggcggc                        >  2:9148/1‑90 (MQ=255)                                                              tCGCCTGGTCTGTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                   >  1:620081/1‑90 (MQ=255)                                                                         gTAAAGGCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAAc        >  2:34726/1‑90 (MQ=255)                                                                               gCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc  >  2:516086/1‑90 (MQ=255)                                                                               gCTGGGAGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc  >  2:629942/1‑90 (MQ=255)                                                                                     |                                                                                    CTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCC  >  NZ_CP009273/1613543‑1613710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑