| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,362 | 0 | C | T | 52.6% | 9.2 / 53.9 | 38 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base C (3/15); new base T (20/0); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.27e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-08 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA > NZ_CP009273/2380275‑2380451 | gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTccc < 2:250640/90‑1 (MQ=255) tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTc < 1:1312812/90‑1 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCACAAAAAACGAACAAG‑ACATTTTTTTTTTCCTcc > 2:1047634/1‑89 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAG‑ACATGCACAATTTTTTTTTCCTcc > 1:506975/1‑89 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAG‑ACAAGCACAATTTTTTTTTCCTCa > 1:440576/1‑90 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAA‑ACAAGCACATTTTTTTTTTCCCCAt > 1:106687/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATc < 2:923029/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATc < 1:698933/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATc < 1:593737/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATc < 1:353816/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATc < 1:581233/90‑1 (MQ=255) gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCt < 2:489476/90‑1 (MQ=255) gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCt < 2:298695/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCAGAAAAAAAA‑AAAAGCACAATTTTTTTTTTCTCCTCTGttttttg > 2:819604/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACG‑AAAAACACAATTTTTTTTTCCCCATCCGtttttta > 1:104564/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAA‑AAAAACACAATTTATTTTTTCTCATCTGttttttg > 2:459481/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAG‑AAAAGAACATTTTTTTTTTTCTCTTCTGTTTTTAg > 2:116844/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAG‑ACATGCACATTTTTTTTTTCCTCTTCtttttttaa > 1:1029004/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACG‑ACAAGCCCAATTTTTTTTTCCTCATCTCtttttaa > 1:1579599/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAG‑ACATGCACATTTTTTTTTTCCTCCTCTGttttttg > 1:1106871/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAG‑ACAAGCACATTTTTTTTTTTCTCCTCtttttttta > 1:1156507/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAAAAAAAAGG‑ACATACACATTTTTTTTTTTCTCCTCTTTTTTTAg > 1:844008/1‑90 (MQ=255) caGTAAATAATAAAAATTTCTCGAGGCGGGGGCCCAAAAAAAG‑ACAAGCACATTTTTTTTTTTCTCTTCTTTTTTTAAAT‑GCGgaaaaaa > 1:96644/1‑86 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAACG‑ACAAGCACAATTTTTTTTTCCTCCTCTTTTTTTAAATGGGGAAAAATAAAAtta > 1:894075/1‑89 (MQ=255) taataaAAATTTCTCGAGGCGGGCCCCAAAAAAAAG‑AAAAGGCCAATTTTTTTTTCCTCCTTTGTTTTTAAAA‑GCGGAAAAAGaaaattt > 1:183661/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAG‑ACAAGCACATTTTTTTTTTCCTCTTCTGTTTTTATAT‑GCGAAAAAAGAAAATCaaa > 2:125816/1‑89 (MQ=255) cagtctcGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACggagga < 1:330933/87‑1 (MQ=255) ttCTCGAGGCGGGTCCCGAAAAAAAG‑AAAAGCACATTTTTTTTTTTCTCCTCTGTTTTTAAAA‑GGGGAAAAAGAAAATCAAAGGAGGaaa > 2:438094/1‑90 (MQ=255) ttCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGaaa < 1:683044/90‑1 (MQ=255) ttCTCGAGGCGGGGCCAGAAAAAAAG‑ACAAGCACATTTTATTTTTTCTCATCTTTTTTTAGAT‑GAGAAAAAAGAAAATCAAACGAGGaaa > 2:402686/1‑90 (MQ=255) tCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAt < 1:1615570/90‑1 (MQ=255) ccAGAAAAAGCG‑ACATGCACATTTTATTTTTCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATg > 2:397469/1‑90 (MQ=255) aaaaaGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTg < 2:457233/90‑1 (MQ=255) ttttattttCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt < 1:927604/90‑1 (MQ=255) ttttattttCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt < 1:1537532/90‑1 (MQ=255) tattttCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTTCAGAAATTGAc > 2:1518307/1‑90 (MQ=255) tattttCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGAc > 1:535287/1‑90 (MQ=255) attttCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt < 1:980565/90‑1 (MQ=255) tCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa > 1:636600/1‑90 (MQ=255) cccTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa < 1:399126/90‑1 (MQ=255) cccTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa < 1:438094/90‑1 (MQ=255) | GATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCG‑ACATGCACATTTTATTTTCCCTCATCTGTTTTAAGAT‑GCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA > NZ_CP009273/2380275‑2380451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |