| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,436,061 | 0 | T | G | 61.1% | 6.8 / 11.5 | 18 | G273G (GGT→GGG) | mnmC | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (11/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCG > NZ_CP009273/2435979‑2436140 | aGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGGTTGccc > 2:547081/1‑89 (MQ=255) tACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCAGcgcgg > 1:336284/1‑89 (MQ=255) cTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGGCAGCGCGgtgttg > 1:1369465/1‑90 (MQ=255) cTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTGCCAGCCCGgtggtg > 1:745598/1‑90 (MQ=255) ccccTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTTCCAGCGCgttgttttt > 1:1167197/1‑87 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGGTTTCCCGCGCGGTGTTGTCGCTGGCGCTa > 2:663197/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGGTGTTGTGGCCTGCGCTa > 1:215349/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCCGCGCGGTGGTTTTGCTGGCGCTa > 1:1323500/1‑90 (MQ=255) gcgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGGGGGATTTCCAGCGCGTTGTTTTCGCTGGGGCTa > 1:280183/1‑90 (MQ=255) cACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTTCCAACGCGTTGTTGTCGCTGGCGCTCTTACGGCGCGGCTggtc > 1:1255713/1‑88 (MQ=255) cACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGGATTTCCCGCGCGGTGTTTTCGCTGGCGCTATTTCGGCGCGGCTgggg > 1:895050/1‑88 (MQ=255) gcagcaAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGc < 1:704984/90‑1 (MQ=255) aaCGGGAAGCGGCGATTATCGGCGGTGGGATTGCCAGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGGAGGGAAAGCCTtttt > 1:1242049/1‑90 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 2:1167197/90‑1 (MQ=255) ggAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcgc < 2:1323500/90‑1 (MQ=255) gcgATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGGAGGTAACGCTTTATTGCGCGGATGAg < 1:1203558/90‑1 (MQ=255) gcgATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAg < 2:1498593/90‑1 (MQ=255) tCGGCGGTGGGATTTTCCGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGGTGGCGGGGAAAGCTTTTTTTTGCGGGTGGGGgccccc > 1:539926/1‑89 (MQ=255) | AGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCG > NZ_CP009273/2435979‑2436140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |