| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 52.2% | 16.5 / 32.0 | 23 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (11/0); new base C (0/12); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAA > NZ_CP009273/3214050‑3214215 | aTCGGATATACCTTGGCCAGGGAGTTTTCCCGCCAGCGCGT‑CCGGGGGCCGGAAACGCTAAAAAACACAAAAACGACCCGCATTGAACCg < 2:973847/90‑1 (MQ=255) tttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactg > 1:875585/1‑90 (MQ=255) tttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactg > 2:1309251/1‑90 (MQ=255) ttggCCAGGGGGTTGTCCCGCCGGCGCGTACGGGGGGCCGA‑ACCCCTAAAAAACCCAAAAACGACCCGAATTAACCCGCcactgacactg < 2:729367/87‑1 (MQ=255) ttggCCAGCGAGATGTCCCGCCAGCGCGTCCGGGGGGCGGG‑ACCCCACAACAACCCAAAAACGACCCGCATTAATCCGCcactgacactg < 1:1396754/87‑1 (MQ=255) ttcgCCAGCGAGATGTCCCCCCGGCCCGTAGGGGGGCCCGA‑ACCCCTAAACAACAAAAAAACGCCCCACATTGAACCGCcactgacactg < 2:1305504/87‑1 (MQ=255) ttccccAGGGAGATGTCCCCCCGCCGCGTCCTGGGGGCCGA‑AACTCCCAATACCCCAAAAACGCCCCGCATTAAGCCGCcactgacactg < 1:363897/86‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:688576/1‑90 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 1:132181/1‑90 (MQ=255) cggggagtTGTCCCCCCAGCGCGTCCGGGTGGCCGA‑ACCGCAAAAAACCCCAAAAACGACCCGAATTAAACCGCCACTGACACTGACCAt < 1:127934/86‑1 (MQ=255) gCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTg > 2:323126/1‑90 (MQ=255) agttGTCCCGCCCGCGCGTTGGGGGGGCGGA‑AACGATAAACACCCCAAAACCTACCCGCATGAAACCGCCACTGACACTGACCATTGAAc < 1:1392180/87‑1 (MQ=255) agaTGTCCCGCCAGCGCGTACTGGGGGCCGG‑AACGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:1492/90‑1 (MQ=255) gaTGTCCCGCCAGCGGGTACTGGGGGCCGG‑AACCCTAAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACa < 1:740899/90‑1 (MQ=255) aGCGCGTACTGGTGGCCGG‑AACGCTCAATAAAGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 1:157848/1‑90 (MQ=255) cgcgTAGTGGGGGCCGA‑AACGCACAAAACCGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg < 1:804329/90‑1 (MQ=255) acgggtggCCGG‑AACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCaa < 2:1018211/87‑1 (MQ=255) ggtggCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgc > 1:961000/1‑90 (MQ=255) acccTCAATAGCCCCAAAACGATCAGCATAGCATCCCCACTGACACTGACCATTGAACAGTGAGAACTGGTGATCAAAGCGGCTCGTAAg > 2:1443964/4‑90 (MQ=255) cAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCt > 1:987912/1‑90 (MQ=255) caaaacCGACCCCCATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 2:598291/88‑1 (MQ=255) aTCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTa > 2:414283/1‑90 (MQ=255) aTCCGCATTGAACCGCCACTGACACTGACCATAGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGACATGCGAGTa > 1:136305/1‑90 (MQ=255) tCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTaa < 1:414283/90‑1 (MQ=255) tCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTaa > 2:1230685/1‑90 (MQ=255) | ATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGG‑AACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAA > NZ_CP009273/3214050‑3214215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |