Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,765	0	C	T	100.0%	229.6 / NA	70	intergenic (+134/‑58)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/32);  total (38/32)

TTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGC  >  NZ_CP009273/49679‑49848                                                                                       |                                                                                    ttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgac                                                                                  <  2:13459/90‑1 (MQ=255)   cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                                <  2:264792/90‑1 (MQ=255)   cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                                <  1:151475/90‑1 (MQ=255)       gTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                                            >  2:1528303/1‑90 (MQ=255)       gTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGt                                                                            >  2:409741/1‑90 (MQ=255)         aacgcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                          >  1:12200/4‑90 (MQ=255)         aaTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                          <  2:577480/90‑1 (MQ=255)          aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTa                                                                         <  2:1406711/90‑1 (MQ=255)            gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                       <  1:134190/90‑1 (MQ=255)             cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                      >  1:647473/1‑90 (MQ=255)              ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                     <  1:344463/90‑1 (MQ=255)              ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                     >  2:1434000/1‑90 (MQ=255)              ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                     <  1:1434000/90‑1 (MQ=255)               gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt                                                                    <  2:813750/90‑1 (MQ=255)               gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt                                                                    <  2:1071435/90‑1 (MQ=255)               gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt                                                                    <  2:1074122/90‑1 (MQ=255)               gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCtt                                                                    <  2:869738/90‑1 (MQ=255)                 gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTa                                                                         >  2:544804/1‑83 (MQ=255)                 gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTa                                                                         <  1:544804/83‑1 (MQ=255)                 gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                                                  <  2:647473/90‑1 (MQ=255)                 gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                                                  <  2:19723/90‑1 (MQ=255)                 gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                                                  >  1:1292782/1‑90 (MQ=255)                  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAc                                                                 >  2:1072819/1‑90 (MQ=255)                            gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                     >  2:331488/1‑76 (MQ=255)                            gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                     <  1:331488/76‑1 (MQ=255)                             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCg                                                      >  2:51931/1‑90 (MQ=255)                             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCg                                                      >  1:818392/1‑90 (MQ=255)                             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCg                                                      >  2:2263/1‑90 (MQ=255)                             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGGTGACGACGGTTTACGCTTTACGCATAGTGGCg                                                      >  2:1025666/1‑90 (MQ=255)                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                       >  1:571965/1‑72 (MQ=255)                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                       <  2:571965/72‑1 (MQ=255)                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGa                                                     >  2:1236738/1‑90 (MQ=255)                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGa                                                     >  2:1361349/1‑90 (MQ=255)                                gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                       <  1:560500/70‑1 (MQ=255)                                gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                       >  2:560500/1‑70 (MQ=255)                                 cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                  >  2:831425/1‑90 (MQ=255)                                  gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt                                                 >  2:1164034/1‑90 (MQ=255)                                  gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt                                                 >  2:515562/1‑90 (MQ=255)                                   tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                                <  1:983327/58‑1 (MQ=255)                                   tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacga                                                                                >  2:983327/1‑58 (MQ=255)                                         cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                                                  >  2:403791/1‑66 (MQ=255)                                         cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTa                                                                  <  1:403791/66‑1 (MQ=255)                                           ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTAt                                        <  2:919543/90‑1 (MQ=255)                                                 gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa                                  <  1:515562/90‑1 (MQ=255)                                                   atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt                                >  2:1221005/1‑90 (MQ=255)                                                   atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt                                >  2:1271690/1‑90 (MQ=255)                                                   atatAAAATTTTCCTCAACAACATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAATGGCGACGATTTTTTTTATCGGGAAAt                                >  2:326214/1‑90 (MQ=255)                                                     ataAAATTTTCCTCAACATCATCCTCCCTCCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAtct                              <  1:1621445/90‑1 (MQ=255)                                                       aaaaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCa                            <  2:1187653/90‑1 (MQ=255)                                                       aaaaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCa                            <  2:239402/90‑1 (MQ=255)                                                           ttttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTAGGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGa                        >  1:1007076/1‑90 (MQ=255)                                                             ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                        >  1:893119/1‑40 (MQ=255)                                                             ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                        <  2:893119/40‑1 (MQ=255)                                                             ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                      >  2:1243014/1‑90 (MQ=255)                                                             ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                      <  1:1238588/90‑1 (MQ=255)                                                             ttCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                      <  1:34989/90‑1 (MQ=255)                                                               ccTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTATCGGGAAATCTCAATGATCAGt                   >  2:546102/1‑90 (MQ=255)                                                                         atcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg          >  2:1445987/1‑90 (MQ=255)                                                                         atcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg          >  2:1513271/1‑90 (MQ=255)                                                                         atcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg          >  2:1326849/1‑90 (MQ=255)                                                                          tcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg         <  1:898201/90‑1 (MQ=255)                                                                          tcCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg         <  1:395254/90‑1 (MQ=255)                                                                            cTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg       <  1:1125706/90‑1 (MQ=255)                                                                             tCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt      >  2:1136541/1‑90 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGAc                                                    <  1:914252/43‑1 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGAc                                                    >  2:914252/1‑43 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt     >  1:430848/1‑90 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt     <  1:2263/90‑1 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt     >  1:160006/1‑90 (MQ=255)                                                                              cGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt     <  2:160006/90‑1 (MQ=255)                                                                               gCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTG‑GGCGTTAg   >  1:793399/1‑90 (MQ=255)                                                                                cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg   >  2:326513/1‑90 (MQ=255)                                                                                 aCCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc  <  1:1221005/90‑1 (MQ=255)                                                                                    aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTAt                                        >  1:1264879/1‑49 (MQ=255)                                                                                    aGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTAt                                        <  2:1264879/49‑1 (MQ=255)                                                                                       |                                                                                    TTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGC  >  NZ_CP009273/49679‑49848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑