Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,858,499	G→C	W131C (TGG→TGC)	yeaD →	D‑hexose‑6‑phosphate mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,858,499	0	G	C	100.0%	156.1 / NA	42	W131C (TGG→TGC)	yeaD	D‑hexose‑6‑phosphate mutarotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/21);  total (21/21)

TGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC  >  NZ_CP009273/1858413‑1858583                                                                                       |                                                                                     tGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCg                                                                                   >  2:309727/1‑90 (MQ=255)   gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCa                                                                                 >  1:1521403/1‑90 (MQ=255)    aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  1:1344684/90‑1 (MQ=255)    aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  2:1320981/90‑1 (MQ=255)              cacaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGc                                                                      <  1:242183/90‑1 (MQ=255)                  tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt                                                                  <  1:1431563/90‑1 (MQ=255)                  tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGtt                                                                  <  2:1483891/90‑1 (MQ=255)                   catGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTa                                                                 >  2:830506/1‑90 (MQ=255)                    atGAAGATGCTGATGGCGTAGCGCTGACTCTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAg                                                                >  2:236658/1‑90 (MQ=255)                          aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt                                                          >  2:630536/1‑90 (MQ=255)                            gCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTc                                                        >  1:1508597/1‑90 (MQ=255)                             cTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTcc                                                       >  1:1267257/1‑90 (MQ=255)                              tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                >  2:611871/1‑64 (MQ=255)                              tGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  1:611871/64‑1 (MQ=255)                                aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  1:331116/90‑1 (MQ=255)                                aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  2:902199/90‑1 (MQ=255)                                 tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                   <  2:764395/90‑1 (MQ=255)                                 tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                   <  2:850791/90‑1 (MQ=255)                                 tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                   <  2:1238824/90‑1 (MQ=255)                                     gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGt                                               >  2:706738/1‑90 (MQ=255)                                     gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGt                                               >  1:985546/1‑90 (MQ=255)                                        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTaaa                                            <  1:630536/90‑1 (MQ=255)                                         cgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTaaaa                                           >  2:948436/1‑90 (MQ=255)                                          gcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAAc                                          <  1:1625653/90‑1 (MQ=255)                                             gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTtg                                       >  2:197973/1‑90 (MQ=255)                                               cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTtgtg                                     <  1:877286/90‑1 (MQ=255)                                                  ttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGaaa                                  >  2:1503307/1‑90 (MQ=255)                                                         gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                           >  1:1119983/1‑90 (MQ=255)                                                         gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                           >  2:1150924/1‑90 (MQ=255)                                                         gACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCt                           >  1:775167/1‑90 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    >  2:1047160/1‑55 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  1:972916/55‑1 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  1:882022/55‑1 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    >  2:882022/1‑55 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  1:1047160/55‑1 (MQ=255)                                                                   gAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    >  2:972916/1‑55 (MQ=255)                                                                        gaCGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGa            <  1:702622/90‑1 (MQ=255)                                                                             aaaaaTTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTg       >  2:1613381/1‑90 (MQ=255)                                                                                  ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  >  2:242241/1‑90 (MQ=255)                                                                                  ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  2:772889/90‑1 (MQ=255)                                                                                  ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  1:1364943/90‑1 (MQ=255)                                                                                  ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  1:1348463/90‑1 (MQ=255)                                                                                       |                                                                                     TGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC  >  NZ_CP009273/1858413‑1858583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑