Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,858,499	G→C	W131C (TGG→TGC)	yeaD →	D‑hexose‑6‑phosphate mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,858,499	0	G	C	100.0%	136.2 / NA	37	W131C (TGG→TGC)	yeaD	D‑hexose‑6‑phosphate mutarotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/21);  total (16/21)

GTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC  >  NZ_CP009273/1858412‑1858583                                                                                        |                                                                                     gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGccc                                                                                    <  2:1244296/90‑1 (MQ=255)     aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  2:942521/90‑1 (MQ=255)     aCGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  1:495498/90‑1 (MQ=255)      cGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACg                                                                               >  2:138708/1‑90 (MQ=255)        cTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc                                                                             >  2:439044/1‑90 (MQ=255)        cTGAAATCACATCATGAAGATGCTGATGGAGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc                                                                             >  1:1729174/1‑90 (MQ=255)        cTGAAATCACATCATGAAGATGCTGATGGAGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGAc                                                                             >  1:407402/1‑90 (MQ=255)             aTCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTAc                                                                        <  1:1155742/90‑1 (MQ=255)              tCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg                                                                       <  2:94279/90‑1 (MQ=255)              tCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg                                                                       <  1:2061617/90‑1 (MQ=255)              tCACATCATGAAGATGCTGATGGAGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg                                                                       <  2:407402/90‑1 (MQ=255)              tCACATCATGAAGATGCTGATGGAGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACg                                                                       <  2:1729174/90‑1 (MQ=255)               cacaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGc                                                                      <  2:723810/90‑1 (MQ=255)                acaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCt                                                                     >  1:367505/1‑90 (MQ=255)                  atcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGt                                                                   >  1:1558483/1‑90 (MQ=255)                      tGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                >  1:2118329/1‑73 (MQ=255)                      tGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  2:2118329/73‑1 (MQ=255)                        aaGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAgcgc                                                             <  2:22820/90‑1 (MQ=255)                           aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt                                                          >  2:622941/1‑90 (MQ=255)                           aTGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCAtt                                                          >  2:491239/1‑90 (MQ=255)                                gATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgc                                                     <  1:742028/90‑1 (MQ=255)                                 aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCcgcg                                                    <  2:514346/90‑1 (MQ=255)                                      gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                   >  1:505979/1‑86 (MQ=255)                                      gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGt                                                   <  2:505979/86‑1 (MQ=255)                                       tAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTa                                              >  2:7667/1‑90 (MQ=255)                                         gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTaaa                                            >  2:1833161/1‑90 (MQ=255)                                          cgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTaaaa                                           <  1:138708/90‑1 (MQ=255)                                             tGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCTCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACtt                                        >  2:1854794/1‑90 (MQ=255)                                              gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                <  2:2070784/49‑1 (MQ=255)                                              gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCAc                                                                                >  1:2070784/1‑49 (MQ=255)                                              gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTtg                                       >  1:78420/1‑90 (MQ=255)                                                     gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATc                                <  1:1090365/90‑1 (MQ=255)                                                     gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATc                                <  2:1616204/90‑1 (MQ=255)                                                        ttGACGCAAAGCGAAGAGACGAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGAt                             <  1:1636272/90‑1 (MQ=255)                                                                            gAAAAAATTCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt         >  1:15657/1‑90 (MQ=255)                                                                                   ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  1:439044/90‑1 (MQ=255)                                                                                   ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  2:576494/90‑1 (MQ=255)                                                                                   ttCTGCCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc  <  1:622941/90‑1 (MQ=255)                                                                                        |                                                                                     GTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACC  >  NZ_CP009273/1858412‑1858583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑