Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,659,0620CA52.9% 4.8 / 21.2 17A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (0/9);  total (5/12)
Fisher's exact test for biased strand distribution p-value = 9.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAC  >  NZ_CP009273/3658973‑3659138
                                                                                         |                                                                            
gAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGc                                                                              >  2:252860/1‑90 (MQ=255)
 aGCATCGGGAGAAATAATGAAAGCGTGGCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                             >  1:235885/1‑90 (MQ=255)
  gCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaa                                                                            <  2:65275/90‑1 (MQ=255)
     tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                         >  2:283886/1‑90 (MQ=255)
     tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                         >  2:166700/1‑90 (MQ=255)
                aatGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTCAACACACTTATCTACTCAATTTTGCCTTGCACCCGAAAAACCCAGGTCACCg                                                              <  1:54847/90‑1 (MQ=255)
                         gtgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaaa                                                     >  1:30466/1‑90 (MQ=255)
                             cccTTGACACAAATAAAAAATTTTCAACCCACTTATCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGc                                                 <  2:255913/90‑1 (MQ=255)
                                    cacaAATAAAAAATTTTCAACACCCTTATCTCCTCAAATTTGGCTTGCATCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc                                          <  2:78351/90‑1 (MQ=255)
                                          tACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                    <  1:197475/90‑1 (MQ=255)
                                               aaTTTTAAACACACTTATCTACTAAATTTTGGCTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCa                               <  1:238379/90‑1 (MQ=255)
                                                    taaaCACATTTATCTACTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                          <  2:4974/88‑1 (MQ=255)
                                                    tCAACACTCTTATATCCTCAATTTTGCTTTCCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                          <  1:226613/90‑1 (MQ=255)
                                                         ccatttATCTACTAAATTTTGGCTTCCCCCCAAAAAAACCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                     <  1:252860/86‑1 (MQ=255)
                                                         actcTTATCTACTAAATTTTGGTTCCCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                     <  1:239268/87‑1 (MQ=255)
                                                         acacTTATCTCCTCGATTTTGGCTTCCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                     <  2:136289/90‑1 (MQ=255)
                                                                    cTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc          >  2:13982/1‑90 (MQ=255)
                                                                            tGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc  <  2:19231/90‑1 (MQ=255)
                                                                                         |                                                                            
GAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAC  >  NZ_CP009273/3658973‑3659138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: