| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,390,909 | 0 | A | C | 57.1% | 6.3 / 11.7 | 14 | G82G (GGT→GGG) | rng | ribonuclease G |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATAT > NZ_CP009273/3390834‑3390979 | ccccTGCACCATCAGATCTTCCCCCTGACGAACCAGTTCCGAGATGTCGCGCCCCGTGAATTGCTTTTGTTCTTCCCCCGCCACACATTc < 1:239241/88‑1 (MQ=255) ccTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCgg > 2:181442/1‑90 (MQ=255) tGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGgtg > 1:96745/1‑90 (MQ=255) cccATCAGTTCTTCCCCCTGACGACCCATTCCCGAGATGTCGCGCCCCGTAATTTGTTTTTTTTCTCCCCCCGCCACACATTCGGTGTGc < 2:225188/89‑1 (MQ=255) aTCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGc > 2:123268/1‑90 (MQ=255) cAGATTTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCCCCGTGAATTGTTTTTTTTCTTCACCCGCCACACATTCGGTGTGCGGCat < 1:26658/90‑1 (MQ=255) tccggagaTGCCCCGCCCCGTGATTTTTTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc < 2:243414/86‑1 (MQ=255) tcccGAGATGTCGCGCCCCGTGAATTGCTTTTGTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc < 1:104460/88‑1 (MQ=255) tcccGAGATGTCGCGCACCGTGAATTGCTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc < 1:260307/88‑1 (MQ=255) tcccGAGATGTCGCCCCCCGTAATTTTTTTTTTTTCTCCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc < 1:623089/88‑1 (MQ=255) tcccGAGAGGTCGCCCCCCGTAATTTTTTTTTTTTCTCCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcgc < 2:177630/88‑1 (MQ=255) gTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATc > 1:536729/1‑90 (MQ=255) gTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATc > 1:655861/1‑90 (MQ=255) tGATTTGTTTTTTTTCTTCCCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAatat < 1:641299/90‑1 (MQ=255) | CACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATAT > NZ_CP009273/3390834‑3390979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |