| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,907,102 | 0 | G | . | 25.0% | 34.7 / 12.0 | 16 | intergenic (‑74/+88) | glmS/glmU | glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)/bifunctional UDP‑N‑acetylglucosamine diphosphorylase/glucosamine‑1‑phosphate N‑acetyltransferase GlmU |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base . (2/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGG > NZ_CP009273/3907026‑3907174 | caTAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTAt > 2:431822/1‑90 (MQ=255) ttttGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTtat > 1:65656/1‑90 (MQ=255) cGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgggagg < 1:537562/90‑1 (MQ=255) gTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgggaggg > 1:315492/1‑90 (MQ=255) gTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTgggaggg > 1:609731/1‑90 (MQ=255) cGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGAtt > 2:173108/1‑90 (MQ=255) ggTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTa < 1:431822/90‑1 (MQ=255) tATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATctcc > 2:163206/1‑90 (MQ=255) tGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATctcctc < 1:163206/90‑1 (MQ=255) tttttCGGGCGCCCCGAGCCTTGT‑‑AGAGTGGGGTTATTTTTATAGTTACTGCTtg < 1:440460/55‑1 (MQ=255) tttttCGGGCGCCCCGAGCCTTGT‑‑AGAGTGGGGTTATTTTTATAGTTACTGCTtg < 1:646402/55‑1 (MQ=255) tttttCGGGCGCCCCGAGCCTTGT‑‑AGAGTGGGGTTATTTTTATAGTTACTGCTtg > 2:440460/1‑55 (MQ=255) tttttCGGGCGCCCCGAGCCTTGT‑‑AGAGTGGGGTTATTTTTATAGTTACTGCTtg > 2:646402/1‑55 (MQ=255) cGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGAc < 1:173108/90‑1 (MQ=255) cGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGAc < 2:294875/90‑1 (MQ=255) gcgcCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCgg > 1:36138/1‑90 (MQ=255) | CATAGTTTTTGATTCCGATTTATATCGTTGTCGGTCAACCTGTATGCCCGTTTTTTCGGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGG > NZ_CP009273/3907026‑3907174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |