| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,926 | 0 | A | C | 57.1% | 4.4 / 13.6 | 14 | G219G (GGT→GGG) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAG > NZ_CP009273/4527840‑4528011 | aaaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑t > 1:108872/1‑90 (MQ=255) attaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTAtt > 1:263214/1‑90 (MQ=255) ccGGTAAAATCAATTTCAAATACGGCACCCGTTCCCATTCTTGTTTGGGCTTCCCCATTAAAAAGCCAGTTTTTACCCCC‑TTTATC‑caca < 2:567969/90‑1 (MQ=255) ccGGTAAAATAAATTTAAAATACGGCATCCTTTCGCAATCCTGTTTTGGCTTCGCCATTAAAAAGCCAGTTTTTATCCCC‑TTTATT‑caca < 2:236853/90‑1 (MQ=255) aaaataaatTTAAAATCCGCCATCCGTTCCCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGTTTTTATCCCT‑TTTATT‑CACAAccgc < 2:519337/89‑1 (MQ=255) atccGGCACCCGTCCCCAACCCTGTTTTGGCTTCGCCATAAAAAAGCCGGTTTTTATCCCT‑TTTATT‑CACAACCGCCGCACCAGCCGTTc < 1:470232/87‑1 (MQ=255) cctgggTTTGGC‑TCCCCAATAAAAAACCAAGTTTTTACCCC‑TTTTTTCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 1:455645/86‑1 (MQ=255) tcttgTTTGGCTTCCCCATTAAAAAAGCCGGTTTTTTCCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 2:178457/86‑1 (MQ=255) ttGGCTTCCCCATTAAAAAACCCGGTTTTTACCCC‑TTTTTTCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa < 2:32477/90‑1 (MQ=255) tgccTTCCCCATAAAAAAGCCAGGTTTTATCCCC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc < 1:120220/87‑1 (MQ=255) ggTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc > 1:311901/1‑90 (MQ=255) ggTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc > 1:512359/1‑90 (MQ=255) ggTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc > 2:454213/1‑90 (MQ=255) tATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAg > 1:408631/1‑90 (MQ=255) | AAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAG > NZ_CP009273/4527840‑4528011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |