Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9990TG60.0% 5.6 / 26.0 25V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/10);  new base G (15/0);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 3.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-06
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC  >  NZ_CP009273/349930‑350084
                                                                      |                                                                                      
tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGc                                                                   <  2:293996/90‑1 (MQ=255)
       gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTG‑AAAGGGGAgggggg                                                            >  1:186573/1‑89 (MQ=255)
       gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGCGCCG‑GAAAGGGAgggggg                                                            >  2:104650/1‑89 (MQ=255)
        cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAGA‑GGGCTG‑AAAGGGGAgggggcg                                                           >  2:503558/1‑90 (MQ=255)
          cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAggtggcggt                                                         <  1:504409/90‑1 (MQ=255)
                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTc                                               <  2:143911/90‑1 (MQ=255)
                    gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTc                                               <  1:566411/90‑1 (MQ=255)
                      gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCAt                                             >  1:158554/1‑90 (MQ=255)
                        gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGGGTTTGTCATTc                                           >  1:95039/1‑90 (MQ=255)
                        gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGCGTTTGTCATTc                                           >  1:14563/1‑90 (MQ=255)
                        gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGCGGGTGGGGGGAAAGATGCGCTG‑AGAGGGGCGGGGGCGGGGGGGTTTGTCATTc                                           >  1:90420/1‑90 (MQ=255)
                           tctcCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGa                                        >  1:251214/1‑90 (MQ=255)
                                aGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAAATGCGCTG‑AAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAagag                                   >  1:284050/1‑90 (MQ=255)
                                aGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGATGCGCTG‑GAAAGGGGGGGGGGGGGGGGGGTTGTCATTTCGGAagag                                   >  2:371105/1‑90 (MQ=255)
                                    aTCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTTTTCTTTCCG‑Aagagaggg                               >  2:206477/1‑88 (MQ=255)
                                     tCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGa                              >  1:474634/1‑90 (MQ=255)
                                      ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:173425/90‑1 (MQ=255)
                                      ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:21566/90‑1 (MQ=255)
                                      ccGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                             <  2:564850/90‑1 (MQ=255)
                                           cGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCt                        >  1:125811/1‑90 (MQ=255)
                                              tGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGGGCTG‑AAAAGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGa                     >  1:623074/1‑90 (MQ=255)
                                                ccGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                   <  2:284050/90‑1 (MQ=255)
                                                       ggC‑GGTGGGTGGGGGGGAAGATGGGCTG‑AAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGGCAGGCTGGAAGAGCgggg            >  2:416374/1‑87 (MQ=255)
                                                                 tGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa   <  1:331789/90‑1 (MQ=255)
                                                                  ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc  <  2:251214/90‑1 (MQ=255)
                                                                      |                                                                                      
TGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAC  >  NZ_CP009273/349930‑350084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑