| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 350,029 | 0 | T | G | 52.2% | 20.2 / 29.8 | 23 | V538G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (2/9); new base G (12/0); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTG > NZ_CP009273/349940‑350111 | cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAggtggcggt < 1:504409/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTc < 2:143911/90‑1 (MQ=255) gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTc < 1:566411/90‑1 (MQ=255) gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCAt > 1:158554/1‑90 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGGGTTTGTCATTc > 1:95039/1‑90 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGCGTTTGTCATTc > 1:14563/1‑90 (MQ=255) gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGAAAGATGCGCTG‑AGAGGGGCGGGGGCGGGGGGGTTTGTCATTc > 1:90420/1‑90 (MQ=255) tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGa > 1:251214/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTG‑AAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAagag > 1:284050/1‑90 (MQ=255) aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTG‑GAAAGGGGGGGGGGGGGGGGGGTTGTCATTTCGGAagag > 2:371105/1‑90 (MQ=255) aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTTTTCTTTCCG‑Aagagaggg > 2:206477/1‑88 (MQ=255) tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGa > 1:474634/1‑90 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:564850/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:21566/90‑1 (MQ=255) ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:173425/90‑1 (MQ=255) cGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCt > 1:125811/1‑90 (MQ=255) tGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTG‑AAAAGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGa > 1:623074/1‑90 (MQ=255) ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg < 2:284050/90‑1 (MQ=255) ggCGGTGGGTGGGGGGGAAGATGGGCTG‑AAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGGGGCAGGCTGGAAGAGCgggg > 2:416374/1‑87 (MQ=255) tGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa < 1:331789/90‑1 (MQ=255) ggggTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc < 2:251214/90‑1 (MQ=255) aaaGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa > 2:196602/1‑90 (MQ=255) gcgcTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc > 1:415321/1‑51 (MQ=255) gcgcTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc < 2:415321/51‑1 (MQ=255) gtggcggtggcgTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg < 1:416374/90‑1 (MQ=255) | CGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTG > NZ_CP009273/349940‑350111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |