| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,912 | G→T | W30C (TGG→TGT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,912 | 0 | G | T | 100.0% | 54.6 / NA | 17 | W30C (TGG→TGT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (9/8); total (9/8) | |||||||||||
GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49825‑49991 | gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGttt < 2:350926/90‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaaa > 1:63324/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 2:178413/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt > 1:36128/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCGGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCCTGATTa > 2:315201/1‑90 (MQ=255) tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg < 1:76655/90‑1 (MQ=255) ggCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc < 1:53504/90‑1 (MQ=255) aTGAAAACCCCCATGCCGTGAACCCTCCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 2:485638/90‑1 (MQ=255) aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 2:63341/1‑90 (MQ=255) tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa < 1:63341/90‑1 (MQ=255) tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa > 2:352168/1‑90 (MQ=255) aCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaataa < 2:420737/46‑1 (MQ=255) aCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaataa > 1:420737/1‑46 (MQ=255) tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt < 2:17498/49‑1 (MQ=255) tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt > 1:17498/1‑49 (MQ=255) tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt > 2:171406/1‑90 (MQ=255) tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt > 2:126149/1‑90 (MQ=255) aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc < 2:63324/90‑1 (MQ=255) | GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAC > NZ_CP009273/49825‑49991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |