| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 429,105 | 0 | T | G | 52.9% | 4.5 / 11.1 | 17 | G65G (GGT→GGG) | ribD | bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATC > NZ_CP009273/429033‑429177 | ttcccaccaACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTcac < 1:497642/87‑1 (MQ=255)ttACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTcac < 1:175685/90‑1 (MQ=255)ttACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTcac < 2:276572/90‑1 (MQ=255) tACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTcaca > 1:443527/1‑90 (MQ=255) aaCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAAc > 1:296441/1‑90 (MQ=255) aaCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGGGCGACCGCCTATGTCGCAATccgac > 1:614399/1‑86 (MQ=255) gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGGGCGACCCCCCATGTCCCACTCGAACCCCGt > 2:640590/1‑90 (MQ=255) gggTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGGGCGACCGCCCATTTCACACCCGAACCCTGTAg > 2:383387/1‑90 (MQ=255) acaTGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCCAACCCCGTAGCCATCATgg > 2:209757/1‑90 (MQ=255) aTGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCCCACTCGAACCCTGTAGCCATCATGGTc > 2:88201/1‑90 (MQ=255) tACACGCTTTGCGTATGGCGGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCAc < 2:296441/90‑1 (MQ=255) tACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCAc < 1:446889/90‑1 (MQ=255) tGCGTATGGCGGGTGAAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGGACCCCGTGGCCAACATTGTCGTACGCCCCCCTGCtgtg > 2:636656/1‑90 (MQ=255) aTGGCGGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGGACGCCACCGGGCTGTGACGCa > 2:97473/1‑90 (MQ=255) ggTGAAAAAGCCAAAGGTGCGACCGCCTATTTCACACTCGAACCCCGTAGCCATCATGGGCGTACGCCACCCTGCTGTGACGCACTCATc > 2:595486/1‑90 (MQ=255) aaGGTGCGACCGCCTATGTCACACTCGAACCCTGTa > 1:602746/1‑36 (MQ=255) aaGGTGCGACCGCCTATGTCACACTCGAACCCTGTa < 2:602746/36‑1 (MQ=255) | TTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATC > NZ_CP009273/429033‑429177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |