| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,080 | 0 | A | C | 57.1% | 6.2 / 12.6 | 14 | V79G (GTG→GGG) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGC > NZ_CP009273/509996‑510166 | aGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACccgc > 2:299059/1‑90 (MQ=255) ttCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACaa > 2:711347/1‑90 (MQ=255) ttCCAGCAAAAACCGTCGGCAAACCAGCGGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTTGTTCGCCCCCCCGCCGCGACaa < 1:112425/90‑1 (MQ=255) tcccAGCAAAAACCGCCGGCCAATCAGCTGCTCCCCGCGCTGTTTTAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACaa < 1:711347/88‑1 (MQ=255) accGTCGGCAAACCAGCTGCCGCCCGCGTTGTTTTAAATGACTTTCGTTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTccacc < 1:555185/88‑1 (MQ=255) gTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGc > 2:61766/1‑90 (MQ=255) tttAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 1:206982/90‑1 (MQ=255) tttAAATCAATATCTTTGTGTTTTTCTGCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:522202/90‑1 (MQ=255) ttaaaaTGTCTCTCGTTGTGTTTTTGTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCa < 2:386617/87‑1 (MQ=255) taaaaTGCCTCCCGCTGTGTTTTTGGCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCaa < 2:598716/88‑1 (MQ=255) aCTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAc > 2:86309/1‑90 (MQ=255) tgtgTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGcc < 1:72618/90‑1 (MQ=255) tttGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTccc > 1:292821/1‑90 (MQ=255) gacACCCGCCGCGACAACCATTTCCGCCACAGCCCGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGc < 2:731482/88‑1 (MQ=255) | AGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGC > NZ_CP009273/509996‑510166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |