| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 53.3% | 6.4 / 13.5 | 15 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAC > NZ_CP009273/822871‑823020 | cTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAggg > 1:286293/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg > 2:29320/1‑90 (MQ=255) tggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCg < 2:229037/90‑1 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtc < 1:653634/90‑1 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGttgtcgg > 1:548175/1‑89 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGaaaa > 1:646312/1‑89 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGa < 2:655472/90‑1 (MQ=255) cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa < 1:29320/90‑1 (MQ=255) cATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATg > 1:234906/1‑90 (MQ=255) aTGGTGTTGATGTGCAGCGAAAATTCACGGTGGGTGGGGGGGTCAGCGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:708796/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGTCGGGGAAAAAAAAGTTCGGGTCATGc > 2:152563/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAAAATGGCCGGGTCATGc > 1:299203/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGTTCGGCGAGAAAAAGAAAGTCCGGGTccttc > 1:320327/1‑86 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAg > 1:200170/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:646312/90‑1 (MQ=255) | CTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAC > NZ_CP009273/822871‑823020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |