| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 883,470 | 0 | T | G | 52.9% | 16.1 / 20.2 | 17 | intergenic (+55/+120) | rcdA/ybjL | DNA‑binding transcriptional regulator RcdA/aspartate:alanine antiporter |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGC > NZ_CP009273/883385‑883549 | tttGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGAt < 1:526753/90‑1 (MQ=255) gTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATgggg < 2:80214/89‑1 (MQ=255) gTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATgggg > 1:80214/1‑89 (MQ=255) gtagtaGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGGCATCAAAg > 1:224058/1‑90 (MQ=255) tagtagATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGGTCCAAAAAAAGGGGGCATaaaaaa > 2:396251/1‑88 (MQ=255) tagATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGaaaa < 1:513967/90‑1 (MQ=255) tAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGGTCCACAAAAAGGGGGCATCAAAAAAAAACAg > 2:124683/1‑90 (MQ=255) tAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAATGGGGAAATCAAAAAAAAGCAg > 1:467254/1‑90 (MQ=255) tAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGACATCAAAGAAAAGCAg > 1:315611/1‑90 (MQ=255) tttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGGTCCACAAAAAAGGGACATCAAAAAAAAACAGTggga > 2:634777/1‑88 (MQ=255) tttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGGCATCAAAAAAAAACAGTGGCa > 2:214340/1‑90 (MQ=255) ttAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCAc < 2:371143/90‑1 (MQ=255) aCAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGGTGATGGGCTCCACAAAAAGGGGACATCAAAAAAAAACAGGGGGACTAAttta > 1:688824/1‑88 (MQ=255) cAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAg < 2:594496/90‑1 (MQ=255) tGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGaaa < 2:315160/90‑1 (MQ=255) aaaTGGCGGGGGTGATGGGCTCCACAAAAAGGGGACATCAAAGAAAAACAGTGGCACTAATTAAAACTGATGCCCTGCGGGAAAGTTCTg > 2:50706/1‑90 (MQ=255) aaTGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGc < 1:50706/90‑1 (MQ=255) | TTTGAGGATGGTTGAGAGGGTTGCAGGGTAGTAGATAAGTTTTAGATAACAAAAAACCCATCAACCTTGAACCGAAATGGCGGGGTTGATGGGCTCCACAAAATGGGGACATCAAAGAAAAGCAGTGGCACTAATTAAGACTGATGCCCTGCGGAAAAGTTCTGC > NZ_CP009273/883385‑883549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |