| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 976,689 | 0 | A | G | 56.2% | ‑0.9 / 11.8 | 16 | I63V (ATC→GTC) | ldtD | L,D‑transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAC > NZ_CP009273/976607‑976778 | aaTCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGcc > 1:646591/1‑90 (MQ=255) aTCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGccc > 2:262935/1‑89 (MQ=255) tCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCAt < 1:270943/90‑1 (MQ=255) tGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGCCCCTGcc > 2:36066/1‑90 (MQ=255) gTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCCTTGCCTGAAGGGGGGGCAGGAAAAAccc > 1:51787/1‑90 (MQ=255) gAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCCGCCCTTTCCTGAAAGGGGGGCAGAAAAAAccccc > 1:724711/1‑88 (MQ=255) aCAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCcgcac < 2:312049/90‑1 (MQ=255) aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGCCCGAAGGGGCGGGAGAAAAAACCcgcacgc > 1:625735/1‑90 (MQ=255) aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGCCATTTCCTGAAAGGGCGGCAGAAAAAACCcgcacgc > 1:607321/1‑90 (MQ=255) aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCCTTGCCTGAAAGGGGGGCAGAAAAAAACcccacgc > 1:576692/1‑90 (MQ=255) aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGGGGGGGAGAAAAAACCcgcacgc > 1:39253/1‑90 (MQ=255) aGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGCCTGAAGGGGGGGCCGAAAAAACCCCCCCGCcaaaag > 1:695421/1‑87 (MQ=255) gCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAACCCCCACGCAAATCGAATCTCAAtt > 1:197954/1‑90 (MQ=255) cAAGCCACGGCAATAATGGCGGGGGTCCAGCCCCTGCCTGAAGGGGCGGCGGAAAAAAACCCCCCGCAAAACGAAACTCAATTAaccccc > 2:543122/1‑87 (MQ=255) cAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCa < 2:576692/90‑1 (MQ=255) aCGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTAc < 1:717201/90‑1 (MQ=255) aTCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAc > 2:117606/1‑90 (MQ=255) aTCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAc < 1:411038/90‑1 (MQ=255) | AATCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAC > NZ_CP009273/976607‑976778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |