| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 62.5% | 5.7 / 12.0 | 16 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (10/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141135‑2141302 | aCCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTGGTGGCGGGAAAACG‑CAtt > 2:146217/1‑90 (MQ=255) gCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCCATTTgt > 1:310436/1‑89 (MQ=255) tattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCG‑CATTTGCTAAAAATGGaa > 1:118975/1‑90 (MQ=255) gtTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAg < 1:384511/90‑1 (MQ=255) tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCAc < 2:118975/90‑1 (MQ=255) tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCC‑CATTTTCTAAAAAAGGGAAAAAACAGCAc > 1:672382/1‑90 (MQ=255) tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACG‑CATTTTTTAAAAAAGGAAAAAAAAAGCAc > 2:234976/1‑90 (MQ=255) aCGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAAACC‑CCTTTTTTAAAAAAAGAAAAAGACAACACGCCGCTGCCCTCaa > 2:458031/1‑90 (MQ=255) gCAAACTGTTCAGTGGCGGTCATTCGGGGCGGGAAACCG‑CACTTTCTAAAAAAGGAAAAAGACAACACCCCGCTGCCTTCAATGCttttt > 2:618081/1‑88 (MQ=255) gCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCG‑CATTTTCTTAAAATTGAAAAAGACAGCACGCTGCTGCCTTCAATGCttttc > 1:450531/1‑88 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGACGGGAAACCG‑CATTTTCTAAAAATGGAAAACGACAGCACGCCGCTGCCTTCAATGCTTTGCGCGcc > 1:285316/1‑90 (MQ=255) cTGTTCAGTGGCGGGTATGCGTGGCGGGAAAACC‑CCTTTTCTTAAAAAGGAAAAAGACAGCACGCCGCTGCCCTCCATGCTTTTTGCGcc > 2:543094/1‑90 (MQ=255) gTGGCGGTCATGCGTGACGGGAAAACC‑CCTTTTCTAAAAAAGGAAAAAGACAGCACCCCGCTGCCCTCAATGCTTTTCGCGCCCAcgcgg > 2:610590/1‑89 (MQ=255) aCGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGa > 1:338305/1‑76 (MQ=255) aCGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGa < 2:338305/76‑1 (MQ=255) gTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGc > 1:317483/1‑90 (MQ=255) | ACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCG‑CATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141135‑2141302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |