| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,466 | 0 | T | G | 58.3% | 1.4 / 17.3 | 24 | V672G (GTG→GGG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (8/2); new base G (0/14); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 | tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 1:154983/1‑90 (MQ=255)tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 2:246324/1‑90 (MQ=255)tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 1:397504/1‑90 (MQ=255) tcgCATTTTCCCCCGCGGGGGCGCGGCAAATGCCCGGGCGCCGGGGCCCTCACCTTTTATGGGGGAGATGACTGAAACCGCCAATAtttt < 2:142978/90‑1 (MQ=255) tcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATAtttt > 1:519191/1‑90 (MQ=255) tCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATa > 1:664301/1‑90 (MQ=255) tCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATa > 1:457432/1‑90 (MQ=255) tccccGCGTGGGCGCGGAAGATGCCCTGGCGCCCGGGCGCTCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:559868/88‑1 (MQ=255) tccccGCGTGGGCGCAGAAAATCCCCGGGCGGCCGGCCGCCCAACCTTTATGGGGGAGATAACTGAAACCGCCAATATTTTACATAACGc < 2:381579/88‑1 (MQ=255) tccccGCGTAGGCGGGGCAGCTGCCCTGGCGTCCGGGCCCCAACCCTTTGTGGGGGAGATGACTAAACCCGCCAATATTTTACATAACGc < 2:457432/88‑1 (MQ=255) tccccGCGGAGGCGCGGCAGATGCCCGGGCGTCGGGCCCCTAACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 2:598392/88‑1 (MQ=255) tccccGCGAGGGCGCGGAAGATGCCCGGGCGCCGGGGCCCTCAACTTTTATGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGc < 1:728408/88‑1 (MQ=255) cccccGGGTGGGCGCGGAGGATGACGTGGCGCCGGGGCCCTCACCCTTTAGGGTGGAGATGACTGAACCCGCCAATATTTTACATAACGc < 1:420566/88‑1 (MQ=255) cgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGa > 1:3655/1‑90 (MQ=255) cgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGa > 1:598194/1‑90 (MQ=255) gggCGCGGCAGATGCCCGGGCGCCGGGGCGCTCACCTTTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATa < 2:215827/89‑1 (MQ=255) gggCGCGGCAGATGCCCGGGCGCCCGGGCCCTCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATa < 2:195597/89‑1 (MQ=255) ggCGCGGCAGATGCCCGGGCGTCCGGGCGCTCACCTTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATAc < 1:362666/90‑1 (MQ=255) cGTCCGGGCGCTCACCCTTTAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATg < 1:122086/90‑1 (MQ=255) gggCGCTCACCTTTTATGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 1:236202/90‑1 (MQ=255) gggCGCTCAACCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:480214/90‑1 (MQ=255) aCCTTTAGGGGGGAGATGATGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGa < 2:243647/90‑1 (MQ=255) ccTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGaa < 1:246324/90‑1 (MQ=255) tATGGGGGAGATGACAAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTc < 2:341681/90‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 1:91098/1‑90 (MQ=255) | TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |