| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,436,636 | 0 | A | C | 53.3% | 1.9 / 16.6 | 15 | V274G (GTA→GGA) | secY | preprotein translocase subunit SecY |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCG > NZ_CP009273/3436553‑3436713 | aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCg > 2:279901/1‑90 (MQ=255) gTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACt > 1:303917/1‑90 (MQ=255) cccccccGACCCAGGGCGCGTGGGCCGCGGAAAAAAAAATAATACGGGAAGAGAAG‑TTGGCGGGTTTCCCCCCCGCCATATTCACTTTCa < 2:399918/88‑1 (MQ=255) cccccccAACCCATGGCGGGGGGGCCGCCGGAAAAAAAAAAATACGGAAAGAAAAGATGGCGGGGATC‑CCCCCCGCCATATTCACTTTCa < 2:27982/88‑1 (MQ=255) cccccccAAACCATGGCGGGAGGGCCGCGGGAAAAAAAATAATACGGGAAGCGAAGATGGCGGGGATC‑CCCCCCGCCATATTCACTTTCa < 2:279902/88‑1 (MQ=255) gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAg > 1:176305/1‑90 (MQ=255) gAACCAGGCCGCGGGGGCCGCGGGAAAAAAAATAATAGGGGAAGGGAAGTTGGCGGGTATC‑CCCCCCGCCATATTCACTTTCAGCGGTaa < 2:370380/90‑1 (MQ=255) gcgATGGCCGCGGGAAAAAAAAAAATCCGGGAAGCAAAGATGGCGGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc < 1:595449/90‑1 (MQ=255) gcgAGGGCCGCGGGAAAAAAAAAAATACTGGAAGCGAAGTTGGCGGGGATT‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc < 1:607660/90‑1 (MQ=255) tGGCCGCGGGAAAAAAAATAATACGGGAAGAGAAGATGGCGGGTATC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg < 2:90552/90‑1 (MQ=255) gAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac > 1:13221/1‑90 (MQ=255) aataatACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg > 1:447722/1‑90 (MQ=255) ggAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg > 2:262093/1‑90 (MQ=255) gggAAGTTGGCCGGGATC‑CCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt < 2:434069/88‑1 (MQ=255) aTTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg > 2:73055/1‑90 (MQ=255) | AGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATT‑ACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCG > NZ_CP009273/3436553‑3436713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |