| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,186,009 | 0 | T | G | 57.1% | 6.9 / 11.1 | 14 | T7P (ACC→CCC) | thiC | phosphomethylpyrimidine synthase ThiC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAG > NZ_CP009273/4185934‑4186095 | gTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGGAg > 1:372447/1‑90 (MQ=255) tGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTc < 2:353439/90‑1 (MQ=255) tGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGGAGGTTTTTTTCAGGCATAACTc > 2:590875/1‑90 (MQ=255) ggCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaa < 1:338986/90‑1 (MQ=255) gTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAGCTCATTCCAAAAAg > 1:20056/1‑90 (MQ=255) tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTTCAGACATAACTCATTTCCAAAAGATaaag > 1:413451/1‑88 (MQ=255) gtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTGCAGAAATAACTCATTCCAAAAAATTAAGGACGGGGcc > 2:397754/1‑89 (MQ=255) tCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGGCAGGTTTTTTTCAGACATAACTCATTCCCAAAAAATAAGGAAGTGGGtt > 2:8787/1‑90 (MQ=255) gggCCCGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTTCAGACATAACTCATTCCAAAAAAATAAAGGCGGGGGTTTTTAGACGACggg > 2:296369/1‑89 (MQ=255) ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGAt < 1:325946/90‑1 (MQ=255) cGGGCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAACTCCTTCCAAAAAAATAAGGACGTGGCTTTTTAGACGACGGATggaa > 1:725484/1‑87 (MQ=255) ggCGCGTTGTTCGCGGCGGGGCAGTTTTTTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGGGGCTTGTCAGACGGCGGATGGAGCa > 1:68059/1‑90 (MQ=255) tCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATc < 1:42716/90‑1 (MQ=255) gggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAg < 2:20056/90‑1 (MQ=255) | GTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAG > NZ_CP009273/4185934‑4186095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |