| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,926 | 0 | A | C | 53.8% | 3.5 / 10.9 | 13 | G219G (GGT→GGG) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAA > NZ_CP009273/4527841‑4528009 | aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCtt < 1:312201/90‑1 (MQ=255) ataaaGTTCAAATACGGCATCCGTTCGCAATC‑‑GTTTTGGCTTCGCCATTAATAAGCCAGT‑TTTTATCACCTTTATT‑CACAACCGCCGCAc < 1:9761/87‑1 (MQ=255) taaaaTACGCCACCCGTTCGAAACCCGGTTTTGGCTTCCCCATTAAAAAGCCGGT‑TTTTATCCCTTTTATT‑CACAACCGCCGCACCAGcc < 1:174165/88‑1 (MQ=255) aaTACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGtt > 1:413447/1‑90 (MQ=255) aTACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCCCTTTTATT‑CACAACCGCCGCACCAGCCGTTc < 1:141871/90‑1 (MQ=255) aTCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCa > 2:117378/1‑90 (MQ=255) ccTTGTTTGGCCTCCCCAATAAAAACCCCGGTTTTTACCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 2:81466/90‑1 (MQ=255) ggCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt > 1:699826/1‑90 (MQ=255) ggCTTCCCCATTAAATAACCCGG‑TTTTTCCCCCTTTTTTCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc < 1:644746/90‑1 (MQ=255) ataaataaCCCGGT‑TTTTATCCCTTTTTTC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg < 1:361095/87‑1 (MQ=255) ttAAAAAACCAGGTTTTTACCCCTTTTTTT‑CAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg < 1:651042/90‑1 (MQ=255) gtttttATCCCTTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc < 1:117378/88‑1 (MQ=255) tttATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa > 1:441120/1‑90 (MQ=255) | AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAA > NZ_CP009273/4527841‑4528009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |