| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,884 | C→T | P21L (CCG→CTG) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,884 | 0 | C | T | 88.2% | 36.9 / ‑1.1 | 17 | P21L (CCG→CTG) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (5/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01 | |||||||||||
TTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA > NZ_CP009273/49802‑49969 | tttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAc < 1:241302/90‑1 (MQ=255)tttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc < 1:226902/90‑1 (MQ=255) ttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAAcc < 1:403539/90‑1 (MQ=255) tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGcc < 1:259657/90‑1 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaaa > 2:104447/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCaa < 2:558380/90‑1 (MQ=255) aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 1:386832/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 2:286838/90‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac < 2:724869/85‑1 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac > 1:724869/1‑85 (MQ=255) ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt > 1:596423/1‑90 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt < 1:409825/72‑1 (MQ=255) gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAAGCAACACCt > 2:409825/1‑72 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg > 2:657559/1‑90 (MQ=255) ttACCGGCATGCAAAACCCCATGCTGTGAACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCACCACCTTAAATAAACCCGTGATTATgg < 2:688345/90‑1 (MQ=255) aTGAAAACCGCCATGCTGTGAACCCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 2:596423/90‑1 (MQ=255) aTGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 1:205921/1‑90 (MQ=255) | TTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCA > NZ_CP009273/49802‑49969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |