Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	934,891	A→G	D3G (GAT→GGT)	serS →	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	934,891	0	A	G	92.0%	68.7 / ‑0.3	25	D3G (GAT→GGT)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (14/9);  total (15/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTTACTGTATCCCTGTGGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGATCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGC  >  NZ_CP009273/934815‑934976                                                                             |                                                                                      gttACTGTATCCCTGTGGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATctgctg                                                                          <  2:214381/90‑1 (MQ=255)               gtgGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGa                                                            >  2:470121/1‑90 (MQ=255)               gtgGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGa                                                            >  1:597417/1‑90 (MQ=255)                 ggTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACg                                                          >  2:40051/1‑90 (MQ=255)                      cAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTc                                                     <  2:256732/90‑1 (MQ=255)                         gCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGcc                                                               <  2:668827/77‑1 (MQ=255)                         gCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGcc                                                               >  1:668827/1‑77 (MQ=255)                           tgtgGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGa                                                >  2:702075/1‑90 (MQ=255)                                  acaTCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACt                                          <  1:629250/89‑1 (MQ=255)                                  acaTCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACt                                          >  2:629250/1‑89 (MQ=255)                                  acaTCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGATCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTg                                         >  2:80505/1‑90 (MQ=255)                                   caTCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTgg                                        >  2:306086/1‑90 (MQ=255)                                    aTCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGc                                       <  1:177568/90‑1 (MQ=255)                                      ctcCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACAGGCAc                                     >  2:344014/1‑90 (MQ=255)                                       tcCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACg                                    >  1:64039/1‑90 (MQ=255)                                           aTTTAATTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCgg                                <  1:481322/90‑1 (MQ=255)                                                aTTCGATAAGCACAGGATAAGCATGCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCtt                           >  2:497172/1‑90 (MQ=255)                                                                        gCTCGGTCCCAATCTGCTGCGTAATGAGCCAGAGGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGGAGATAAGCTggg   >  2:528300/1‑90 (MQ=255)                                                                        gCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTggg   >  2:719151/1‑90 (MQ=255)                                                                        gCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTggg   >  2:89213/1‑90 (MQ=255)                                                                        gCTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTggg   >  2:250014/1‑90 (MQ=255)                                                                         cTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGgc  <  2:64039/90‑1 (MQ=255)                                                                         cTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGgc  <  2:597417/90‑1 (MQ=255)                                                                         cTCGGTCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGgc  <  1:26596/90‑1 (MQ=255)                                                                         cTCGATCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGgc  <  1:80505/90‑1 (MQ=255)                                                                             |                                                                                      GTTACTGTATCCCTGTGGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCACAGGATAAGCATGCTCGATCCCAATCTGCTGCGTAATGAGCCAGACGCAGTCGCTGAAAAACTGGCACGCCGGGGCTTTAAGCTGGATGTAGATAAGCTGGGC  >  NZ_CP009273/934815‑934976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑