| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,957 | 0 | A | G | 61.1% | 4.5 / 12.2 | 18 | L494P (CTC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (11/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAA > NZ_CP009273/822886‑823044 | gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg > 2:31356/1‑90 (MQ=255)gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAg > 1:660912/1‑90 (MQ=255) cATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGgtcgtc > 1:324615/1‑90 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAg > 2:15570/1‑90 (MQ=255) cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa < 2:115609/90‑1 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGTCGGGTTGTCGGGAAACAGAAAGTc > 1:391413/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGTTCGTCGGGAAAAAAAAAGTTCGGGTCATGCAt > 1:326749/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGGCGGTTCGTCGAGAAAAAGAATGTTCGGGtcattcatt > 1:226617/1‑89 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCGGAAGGCGGGTCGTCGAGAAACAAAATGTCCGGGTCATGCCTCAg > 1:464534/1‑90 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAAAAAGTCCGGGTCATGCATCAGCg > 2:654300/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:579618/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:555389/90‑1 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAgg > 1:587770/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTCCGGTTCATGCATCAGCGAACAgg > 1:147701/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGTTTGTCGAGAAAAAAAAAGGTCGGGGCTTGCATCAGCGAAAAAgcc > 2:598264/1‑90 (MQ=255) ggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCt < 2:324615/90‑1 (MQ=255) ggcggGGGGGGGGGGGAACGCCGGAAGTCGGGTCGTCGAGAAAAAAAAAGTCCGGGTCATGCATCAGCGAACAGGCCCGCGCCcgccgcc > 2:657081/1‑89 (MQ=255) tGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa < 1:379955/90‑1 (MQ=255) | GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAA > NZ_CP009273/822886‑823044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |