| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 892,811 | 0 | T | G | 52.9% | 10.5 / 17.4 | 17 | V91G (GTG→GGG) | potI | putrescine ABC transporter permease PotI |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCAC > NZ_CP009273/892726‑892889 | aGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTgcgc < 1:375815/90‑1 (MQ=255) tttAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGGGGGCTTTTGGAgggt > 1:231745/1‑88 (MQ=255) gTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTcgcg < 1:309893/90‑1 (MQ=255) gTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTTGCAGGGTTcgcg > 1:170741/1‑90 (MQ=255) cTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGCGTTGGGAGGGTTCGCggg > 2:332655/1‑89 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGGGCTTTGGCAGGGTTTGCGGATc > 1:52832/1‑90 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGGTTTGGCGGGGTTTGCGGGTc > 2:309893/1‑90 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCttt < 2:86092/90‑1 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCttt < 1:539550/90‑1 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTTGCAGGGTTCGCGGGTCAAAAAGCttt > 1:687625/1‑90 (MQ=255) gtgCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTTGGAGGGTTTTCGGGTCAAAAGGGttttgcc > 1:451492/1‑86 (MQ=255) cggcgATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCgcgc < 1:332655/90‑1 (MQ=255) cgATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGcgccgc < 2:30917/90‑1 (MQ=255) aTTGCGGCGGTGGTGCTGGGGCGCTTTGGCAGGTTTCGCGGGTCAAATTGCTTTGCCTTTTTGATCACCGCGCCGCTGGGAATGCCAGAt > 1:567807/1‑90 (MQ=255) gcggcgGTGGTGCTGGTGCGTTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCGCCGCGCCGCTGGTAATGCCAGATGtc < 1:298225/90‑1 (MQ=255) ggcggTGGGGGTGGTGCGCTTTTGCAGGTTTCGCGGGTCAAAAGGGTTTTCCTTTATGATCACCCCGCCGCTGGGAATTCCAGGTGgcat > 2:55062/1‑90 (MQ=255) ggtggtGCTGGGGCGCTTTTGCAGGGTTCCCGGATCAAAAGGCTTTGCCTTTTTGATCACCCCGCCCCTGGTAATGCCAGATGTCATCAc > 2:658828/1‑90 (MQ=255) | AGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCAC > NZ_CP009273/892726‑892889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |