| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,570,334 | 0 | C | A | 53.8% | 7.1 / 20.3 | 13 | D515Y (GAT→TAT) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTC > NZ_CP009273/1570249‑1570409 | gTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGa > 1:662467/1‑90 (MQ=255)gTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGa > 2:334133/1‑90 (MQ=255) cTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGcc < 1:102723/90‑1 (MQ=255) gATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCAt > 2:361512/1‑90 (MQ=255) cgcgTCAGATTTTTATTCCCTGATACCGATCCCGCCCAGCTATTGCGGATATCACGTAACCCCATATAAAAAATATTCCCGCCATAGTaa < 1:113874/90‑1 (MQ=255) gcgTCAGATTTTTATTACCTAATCCCGTTCCCGCCCAGTTATTGGGAATATCACGTAACCCAATAAAAAAAATATTCCCGCCATAGTAAc < 1:699620/90‑1 (MQ=255) gTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACgg > 1:231284/1‑90 (MQ=255) agatTTTTATTCCCTGATCCCGATTCCGTCCAGCTATTGGGGATATCACGTAATCCCATATAAAGAATATTCCCGCCATAGTAACGGTta < 1:207449/90‑1 (MQ=255) ccTGATCCCGATCCCCCCCATTTTTTGGGGATACCACGAAATCCCATAAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTa < 2:121833/90‑1 (MQ=255) cccGCCCAGTTTTTGGGGATATCACGTAACCCCATATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGtt < 1:253875/89‑1 (MQ=255) cGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTg > 2:389569/1‑90 (MQ=255) tCACTTAACCCAATAAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCAt < 1:334133/90‑1 (MQ=255) aCGTAATCCCATAAAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTc < 1:553843/90‑1 (MQ=255) | GTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTC > NZ_CP009273/1570249‑1570409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |