| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,218 | 0 | C | A | 53.8% | 5.7 / 16.2 | 13 | P22T (CCG→ACG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGAC‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG > NZ_CP009273/2141138‑2141300 | ggCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGTATTCGTGGC‑GGTAAACCGCAttttc > 2:557289/1‑88 (MQ=255) tattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTGGC‑GGGAAAACGCATTTTCTAAAAATGGaa > 2:48976/1‑90 (MQ=255) tattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATGCGGGAC‑GGGAAAACGCCTTTGCTAAAAATGGaa > 2:565452/1‑90 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGTCGGGAAAACCCCATTTCTTAAAAAAGGAAAA‑GACAGCACGctgctg > 2:645235/1‑90 (MQ=255) tACGGTACAGCAAACTGTTCAGTGGCGGGCATGCGTGGG‑GGGAAAACGCCATTTTTAAAAAAGGAAAAAGAAAGCCCGCCGCCGCCCTcc > 2:453145/1‑89 (MQ=255) aCGGTACAGCAAACTGTTCAGTGGCGGAATTACGGGGCGGGTAAAAACCATTT‑TTAAAAAAAGAAAAGGACAACAAGCTGCTGGCTTCaa > 2:1382/1‑90 (MQ=255) cAGCAAACTGTTCAGTGGCGGTCATGCGTGGC‑GGGAAAACCCATTTTCTAAAAAAGGAAAAAGACAGCACGCCGCTGCCTTCAATGCttt > 2:119042/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGAC‑GGGAAAACGCATTTGCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTTCGCGcc > 2:660323/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGAC‑GGGAAAACCCATTTTCTAAAAAAGGAAAAAGACAACACGCCGCTGCCTTCAAAGCTTTTCGCGcc > 1:209952/1‑90 (MQ=255) tGTTCAGTGGCGGTCATGCGTGAC‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa < 1:428000/90‑1 (MQ=255) tGGCGGTCATGCGTGAC‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTg < 1:331759/90‑1 (MQ=255) tGAC‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGa < 1:557289/90‑1 (MQ=255) c‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATg < 1:660323/90‑1 (MQ=255) | GGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGAC‑GGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATG > NZ_CP009273/2141138‑2141300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |