| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,371,314 | 0 | G | A | 57.9% | ‑5.2 / 14.4 | 19 | F475F (TTC→TTT) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase |
| Reads supporting (aligned to +/- strand): ref base G (5/3); new base A (0/11); total (5/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTA > NZ_CP009273/2371225‑2371400 | tCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCgag > 1:458461/1‑90 (MQ=255) ggcgTGCAAAAAAGGGACGTTTTGCGGCAAAAAAAAAAACCCCCCCCGCTCGTTTTGCGGGGTTGGCAACAGCGAAAAAATTTGCccgcc < 2:424774/90‑1 (MQ=255) ggcgTCCCCAAAAGGTCCGTTTTGCGGCTTCAGATAGAACCGCCCCCGCGCGCTTTGAGGCGTTGGCAACAGCTAGAAAATTTGCccgcc < 1:650759/90‑1 (MQ=255) gTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCgtt > 1:330009/1‑90 (MQ=255) aaaTGGACTTTTGGCGGCATAAGATAAAACCGCCCCCGCTCGTTTTGCGGCGTTGGCAACAGCAAAAAAATTTGCCCGCCGTTGTTGTTc < 2:547840/90‑1 (MQ=255) ggACTTTTGGCGGCATCAAAAAAAACCGCTCCCGCTCGTTTTGGGGCGTTGGCAACAGCGAGAAATTTTGCCCGCCGTTGTTGTTcacca < 1:297334/90‑1 (MQ=255) ggACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTcacca < 1:281325/90‑1 (MQ=255) gACGTTTTGCGGCATCAGATAGAAACGCTCCCGCTCGCTTTGCGGCGTTGGCAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTcaccac < 1:445650/90‑1 (MQ=255) acAGATACAACCACTCCCGCTCGTTTCGCGGCGTTGGCAACAGGGAAAAATTTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAgcg < 1:534109/89‑1 (MQ=255) ctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTg > 2:232047/1‑90 (MQ=255) ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGc > 1:589177/1‑90 (MQ=255) ggtgTTGGAAACAGGGAAAAATTTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgc < 2:187158/87‑1 (MQ=255) ggcgTTGGCAACAGAAAAAAATTTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAGCCTGACGCAATAACGCCAgc < 2:330009/90‑1 (MQ=255) ggcgTTGGAAACAGCGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAga < 1:361341/90‑2 (MQ=255) ttGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt > 2:199861/1‑90 (MQ=255) ttGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATCAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGt > 1:349813/1‑90 (MQ=255) gCAACAGGGAAAAATTTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga < 2:673135/90‑1 (MQ=255) caaaCAGGGAAAATTTTTACCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga < 1:575924/88‑1 (MQ=255) accAGCAAAAAAATTTCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga < 1:652296/88‑1 (MQ=255) ggagaAATTTCCCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa < 2:482620/89‑1 (MQ=255) cGAAAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa < 1:167549/90‑1 (MQ=255) | TCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTA > NZ_CP009273/2371225‑2371400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |