Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,214,1120TA54.5% 6.1 / 13.1 11N420K (AAT→AAA) ygjGputrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base A (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGC  >  NZ_CP009273/3214024‑3214183
                                                                                        |                                                                        
tGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATa                                                                         <  1:311028/90‑1 (MQ=255)
     aTTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGcc                                                                    >  1:42376/1‑90 (MQ=255)
                                      tttCCCAGAGATATTTTCCCCCAGCGCGTACGGGGGGCGGGAACGCCAAAAAACCCAAAAC‑CGATCCGCATTGAACCGCcactgacactg                                  <  1:412701/90‑1 (MQ=255)
                                      ttccccAGCAAGATGCCCCCCCGGCGCGTACGGGGGGCCGAACCGC‑AAAAAACCCCAAAACCGCCCCCAATAGAACCGCcactgacactg                                  <  2:454147/86‑1 (MQ=255)
                                          ccAGCGAGTTCTCCCCCAAGCGGGTGGGGGGGGCCGGAACCCACAAAAACGCAAAAA‑CATCCCGCATTGAACCGCCACTGACACTGACCa                              <  2:336707/90‑1 (MQ=255)
                                                agaTGTCCCGCCAGCGCGTACTGGGGGCCGGAACGCTAAAAAACCCAAAAA‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc                        <  1:90053/90‑1 (MQ=255)
                                                       ccgccgGCGCGTACTGGTGGCCGAAACCCTAAAAAACCCAAAAA‑CGACCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGa                 <  1:263418/90‑1 (MQ=255)
                                                       ccGCCAGCGCGTACGGGGGGCCGGAACGCTAAAAAACCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGa                 <  1:261250/90‑1 (MQ=255)
                                                         gCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc               >  2:44480/1‑90 (MQ=255)
                                                             gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGt           >  1:169621/1‑90 (MQ=255)
                                                                      ggtggCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgc  >  2:682287/1‑90 (MQ=255)
                                                                                        |                                                                        
TGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGC  >  NZ_CP009273/3214024‑3214183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑